Diversity of fungi cultured from galleries and bodies of ambrosia beetles ( spp.) and carpenter moths () in lenga () forests in Patagonia.

Wood-boring insects play an important role in turnover of trees and biomass in temperate forests and interact with a functionally diverse mycobiome. However, the diversity and dynamics of ambrosia beetles, other wood-boring insects, and their fungi remain relatively poorly understood in the forests of temperate South America. Baseline knowledge of insect and fungal diversity is therefore needed to provide a foundation for understanding the potential future dynamics of these critically important ecosystems in the context of global change. This study aimed to document fungal diversity that could be obtained in culture from larvae, adults, and galleries of ambrosia beetles ( spp.) and a carpenter moth () from lenga () in northwest Patagonia, Argentina. Long molecular barcodes from fungal cultures isolated from galleries, larvae, and adult insects were obtained using nanopore sequencing. Fungal assemblages associated with spp. (32 unique taxa) and (17 unique taxa) differed in structure and composition but shared 11 distinct taxa. Differences were found between fungal assemblages associated with gut tracts and galleries. Fungal assemblages found in galleries and insect bodies of varied among species, seasons, and health conditions of the host crown. Our results also showed that the ophiostomatoid fungi spp. and yeast sp. were commonly found with spp. whereas and sp. were found with . Species of the blue stain fungi , an unidentified sp. and were found with both beetles and moths, and was the most frequently isolated species. This is the first comprehensive study of microbiota isolated from spp. and .