孕期肝脏雌酮和雌二醇葡糖醛酸基转移酶活性。3-甲基胆蒽和苯巴比妥预处理的诱导作用。

Hepatic estrone and estradiol glucuronyltransferase activity in pregnancy. Induction by pretreatment with 3-methylcholanthrene and phenobarbital.

作者信息

Vore M, Soliven E

出版信息

Drug Metab Dispos. 1979 Sep-Oct;7(5):247-51.

Abstract

Hepatic microsomal estrone and estradiol glucuronyltransferase activity were examined in nonpregnant female and pregnant rats and rabbits. Pregnancy decreased glucuronyltransferase activity towards both substrates by 30% in rats and rabbits when activity was expressed per mg of microsomal protein. Because of the increased size of the liver in pregnancy in the rat, activity was increased in this species when expressed per whole liver. The size of the liver was not increased in pregnancy in the rabbit, so that activity per whole liver was also decreased in this species. Pretreatment of nonpregnant rats with 3-methylcholanthrene had little effect on estradiol glucuronyltransferase activity and increased estrone glucuronyltransferase activity 35%. Similar pretreatment of pregnant rats, however, increased estradiol glucuronyltransferase activity approximately twofold and estrone glucuronyltransferase activity approximately threefold. Phenobarbital was a much less potent inducer of estrone and estradiol glucuronyltransferase activities but did increase activities to a greater extent in pregnant rats than in nonpregnant female controls.

摘要

在未怀孕的雌性大鼠和兔子以及怀孕的大鼠和兔子中检测了肝微粒体雌酮和雌二醇葡萄糖醛酸基转移酶的活性。当以每毫克微粒体蛋白表示活性时，怀孕使大鼠和兔子对这两种底物的葡萄糖醛酸基转移酶活性降低了30%。由于大鼠怀孕时肝脏体积增大，以整个肝脏表示活性时，该物种的活性增加。兔子怀孕时肝脏体积没有增加，因此该物种整个肝脏的活性也降低。用3-甲基胆蒽对未怀孕的大鼠进行预处理对雌二醇葡萄糖醛酸基转移酶活性影响不大，而使雌酮葡萄糖醛酸基转移酶活性增加了35%。然而，对怀孕大鼠进行类似的预处理，雌二醇葡萄糖醛酸基转移酶活性增加了约两倍，雌酮葡萄糖醛酸基转移酶活性增加了约三倍。苯巴比妥对雌酮和雌二醇葡萄糖醛酸基转移酶活性的诱导作用较弱，但与未怀孕的雌性对照相比，在怀孕大鼠中确实使活性增加的程度更大。