Lane R W, Simon G S, Dougherty R W, Egle J L, Borzelleca J F
Drug Chem Toxicol. 1985;8(4):265-80. doi: 10.3109/01480548509038650.
Adult male Sprague Dawley rats were gavaged with 2,4-dinitrotoluene (2,4-DNT) dissolved in corn oil at 0, 60, 180, or 240 mg/kg/day for five days. A single oral dose (0.5 mg/kg) of triethylenemelamine was used as a positive control. Induction of dominant lethal events was scored on the basis of early fetal deaths. At the two lower doses, no consistent changes were observed in the numbers of pre-implantation losses, implantation sites, or living or non-living fetuses. The highest dose of 2,4-DNT tested resulted in a marked decrease in the numbers of sperm-positive females (determined by microscopic examination of vaginal smears for sperm) and pregnant females. These two effects diminished in the latter weeks of mating. The low number of pregnant females at the highest dose made meaningful statistical evaluations difficult. The results indicate that 2,4-DNT does not cause dominant lethal mutations but does adversely affect reproductive performance.
成年雄性斯普拉格-道利大鼠连续五天接受灌胃，给予溶解于玉米油中的2,4-二硝基甲苯（2,4-DNT），剂量分别为0、60、180或240毫克/千克/天。单次口服剂量（0.5毫克/千克）的三亚乙基三聚氰胺用作阳性对照。基于早期胎儿死亡情况对显性致死事件进行评分。在两个较低剂量组中，着床前损失、着床部位、存活或死亡胎儿数量均未观察到一致变化。所测试的2,4-DNT最高剂量导致精子阳性雌性大鼠（通过阴道涂片显微镜检查精子确定）和怀孕雌性大鼠数量显著减少。这两种效应在交配后期有所减弱。最高剂量组怀孕雌性大鼠数量较少，难以进行有意义的统计学评估。结果表明，2,4-DNT不会引起显性致死突变，但会对生殖性能产生不利影响。
