Wu Yi-Jun, Kang Kelai, Yang Wenyao, Wu Quangen, Chen Rui, Zhou Xingfan
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichenxilu Road, Beijing, 100101, People's Republic of China.
College of Life and Environment Sciences, Huangshan University, 39 Xihailu Road, Huangshan, 245041, China.
Bull Environ Contam Toxicol. 2025 Aug 4;115(2):24. doi: 10.1007/s00128-025-04097-8.
Cadmium (Cd) is a heavy metal, and chlorpyrifos (CPF) is a widely used organophosphorus insecticide, which are widespread environmental pollutants. These two chemicals can induce the production of reactive oxygen species (ROS) in mammals and cause oxidative damage. However, the mechanism of their combined toxicity in invertebrates and especially insects has not been well documented. In this study, we investigated the combined toxicity of Cd and CPF by using Drosophila S2 cell as a test model. We found that cell viability and antioxidant enzyme activities were reduced and the lipids and proteins peroxidation as well as ROS were induced after the treatment. Cd and CPF displayed an antagonistic interaction in the induction of oxidative damage and the reduction of cell viability. The results indicated that Cd and CPF interfere with the toxicity of each other in the cells, which implies that Cd could influence the effect of CPF used for pest insects control.
镉（Cd）是一种重金属，毒死蜱（CPF）是一种广泛使用的有机磷杀虫剂，它们都是普遍存在的环境污染物。这两种化学物质可在哺乳动物体内诱导活性氧（ROS）的产生并造成氧化损伤。然而，它们对无脊椎动物尤其是昆虫的联合毒性机制尚未有充分记载。在本研究中，我们以果蝇S2细胞为测试模型，研究了镉和毒死蜱的联合毒性。我们发现，处理后细胞活力和抗氧化酶活性降低，脂质和蛋白质过氧化以及ROS增加。镉和毒死蜱在诱导氧化损伤和降低细胞活力方面表现出拮抗作用。结果表明，镉和毒死蜱在细胞中相互干扰毒性，这意味着镉可能会影响用于防治害虫的毒死蜱的效果。
