Amenta Maria Laura, Varriale Stefano, Arbucci Salvatore, Fania Fabio, De Vita Pasquale, Summerer Stephan, Petrozza Angelo, Defez Roberto, Bianco Carmen
Institute of Biosciences and Bioresources (IBBR), Via Pietro Castellino 111, Naples, 80131, Italy.
Institute of Genetics and Biophysics Adriano Buzzati Traverso, Via Pietro Castellino 111, Naples, 80131, Italy.
Sci Rep. 2025 Aug 4;15(1):28348. doi: 10.1038/s41598-025-13827-1.
Plant growth-promoting bacteria (PGPB) represents a sustainable strategy to improve the adaptability of plants to water shortage stress. The present study demonstrated that the endophyte Klebsiella pasteurii BDA134-6, isolated from O. glaberrima rice grown in Mali (West Africa), efficiently colonized the durum wheat variety Primadur. Results obtained from laboratory growth chamber, greenhouse phenotyping platform, and tunnel-type greenhouse demonstrated that inoculated Primadur plants improved their response to water shortage stress. When water shortage stress was simulated by treatment with polyethylene glycol, neither a significant decrease in the relative water content of leaves nor a strong inhibition of acetylene reduction was observed for inoculated plants compared to the uninoculated ones. Inoculated plants subjected to water shortage stress showed increased activity of the antioxidant enzymes glutathione reductase and superoxide dismutase (up to 38% and 191%, respectively), a proline content 3-fold higher, a reduced hydrogen peroxide accumulation (up to 50%), and no significant alterations in lipids peroxidation and leaf senescence. These findings highlight that BDA134-6 is a promising strain. Field trial experiments will allow to evaluate whether it can be used to improve antioxidant defense systems in a plant species different from the native host plant.
植物促生细菌（PGPB）是提高植物对缺水胁迫适应性的一种可持续策略。本研究表明，从生长于马里（西非）的光稃稻中分离出的内生菌巴斯德克雷伯氏菌BDA134 - 6能够有效地定殖于硬粒小麦品种Primadur。从实验室生长室、温室表型分析平台和隧道式温室获得的结果表明，接种了BDA134 - 6的Primadur植株对缺水胁迫的响应得到了改善。当用聚乙二醇处理模拟缺水胁迫时，与未接种的植株相比，接种的植株叶片相对含水量没有显著降低，乙炔还原也没有受到强烈抑制。遭受缺水胁迫的接种植株抗氧化酶谷胱甘肽还原酶和超氧化物歧化酶的活性增加（分别高达38%和191%），脯氨酸含量高出3倍，过氧化氢积累减少（高达50%），脂质过氧化和叶片衰老没有显著变化。这些发现突出表明BDA134 - 6是一种有前景的菌株。田间试验将有助于评估它是否可用于改善不同于原生寄主植物的某一植物物种的抗氧化防御系统。
