从耐旱植物中分离出的植物生长促进细菌有助于小麦植株克服渗透胁迫。

PGPB Isolated from Drought-Tolerant Plants Help Wheat Plants to Overcome Osmotic Stress.

作者信息

Pishchik Veronika N, Chizhevskaya Elena P, Chebotar Vladimir K, Mirskaya Galina V, Khomyakov Yuriy V, Vertebny Vitaliy E, Kononchuk Pavel Y, Kudryavtcev Dmitriy V, Bortsova Olga A, Lapenko Nina G, Tikhonovich Igor A

机构信息

All-Russia Research Institute for Agricultural Microbiology, Podbelskogo Hwy 3, Pushkin, 196608 Saint Petersburg, Russia.

Agrophysical Scientific Research Institute, Grazhdanskiy pr. 14, 195220 Saint Petersburg, Russia.

出版信息

Plants (Basel). 2024 Nov 30;13(23):3381. doi: 10.3390/plants13233381.

DOI:10.3390/plants13233381

PMID:39683174

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11644311/

Abstract

The aim of this research was to study the effect of plant-growth-promoting bacteria (PGPB) isolated from the drought-tolerant plants camel thorn ( (M.Bieb.) Fisch) and white pigweed ( L.) on wheat ( L.) plants cv. Lenigradskaya 6, growing under hydroponic conditions and osmotic stress (generated by 12% polyethylene glycol-6000 (PEG)). Based on the assumption that plants create a unique microbiome that helps them overcome various stresses, we hypothesized that bacteria isolated from drought-tolerant plants may assist cultivated wheat plants in coping with drought stress. PGPB were isolated from seeds and leaves of plants and identified as spp. (strains Cap 07D, Cap 09D, and App 11D); sp. (Cap 286); and sp. (Cap 03D). All bacteria produced different phytohormones such as indole acetic acid (IAA), abscisic acid (ABA), and gibberellic acid (GAS) and were capable of stimulating wheat growth under normal and osmotic stress conditions. All PGPB reduced the malondialdehyde (MDA) content, increased the total chlorophyll content by increasing chlorophyll , and modulated wheat hormone homeostasis and CAT and POX activities under osmotic conditions. Selected strains can be promising candidates for the mitigating of the drought stress of wheat plants.

摘要

本研究的目的是研究从耐旱植物骆驼刺（Alhagi sparsifolia (M.Bieb.) Fisch）和藜（Chenopodium album L.）中分离出的植物促生细菌（PGPB）对水培条件下和渗透胁迫（由12%聚乙二醇-6000（PEG）产生）下生长的小麦（Triticum aestivum L.）品种列宁格勒斯卡亚6号植株的影响。基于植物会形成独特的微生物群落以帮助它们克服各种胁迫这一假设，我们推测从耐旱植物中分离出的细菌可能有助于栽培小麦植株应对干旱胁迫。PGPB从植物的种子和叶片中分离出来，并鉴定为芽孢杆菌属（Bacillus spp.）（菌株Cap 07D、Cap 09D和App 11D）；假单胞菌属（Pseudomonas sp.）（Cap 286）；以及不动杆菌属（Acinetobacter sp.）（Cap 03D）。所有细菌都能产生不同的植物激素，如吲哚乙酸（IAA）、脱落酸（ABA）和赤霉素（GAS），并且能够在正常和渗透胁迫条件下刺激小麦生长。在渗透条件下，所有PGPB都降低了丙二醛（MDA）含量，通过增加叶绿素a来提高总叶绿素含量，并调节小麦激素稳态以及过氧化氢酶（CAT）和过氧化物酶（POX）活性。所选菌株有望成为缓解小麦植株干旱胁迫的候选菌株。