Tchinda Cedric Fossi, Djim-Adjim-Ngana Karyom, Mbogning Therence Annie, Simo Brice Fredy Nemg, Baira Sosthene Boido, Feudjieu Gaizirene Egoume, Matchuenkam Sonia Gayap, Nfor Gael Njini, Takuissu Guy Roussel Nguemto, Essola Kikie Josiane, Walyaro Connie Constance Georgina, Tchamgoue Armelle Deutou
Pharmacology and Drugs Discovery Laboratory, Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, Cameroon.
Laboratory of Microbiology, Infectious Diseases and Immunology, Centre for Research on Health and Priority Pathologies, Institute of Medical Research and Medicinal Plant Studies, Yaoundé, Cameroon.
IJID Reg. 2025 Jul 1;16:100698. doi: 10.1016/j.ijregi.2025.100698. eCollection 2025 Sep.
Carbapenems are critical antibiotics used against multidrug-resistant infections, yet resistance is increasing, especially among Gram-negative bacteria like species. In Cameroon, while is well studied, little is known about other species such as , and . This study assessed the prevalence of carbapenemase-producing and associated risk factors in Yaoundé hospitals.
A 6-month prospective cross-sectional study (April-September 2024) was conducted using 531 clinical samples from four health facilities. Strains were identified via morphologic, biochemical, and API 20 NE® tests. Antibiotic susceptibility was assessed using disc diffusion, and carbapenemases were characterized phenotypically.
Of the samples, 4.9% yielded , with being most common (80.8%), followed by (11.5%) and (7.7%). showed high antibiotic resistance, especially to beta-lactams, with 85.71% producing carbapenemases (83.33% class B and 16.67% class A). Significant risk factors included hospitalization, current antibiotic therapy, recent antibiotic use, and age group 38-47 years.
These findings highlight an urgent need for better surveillance, stricter antibiotic stewardship, and enhanced infection control strategies to limit the spread of resistant infections in Yaoundé's health care settings.
碳青霉烯类是用于对抗多重耐药感染的关键抗生素，但耐药性正在增加，尤其是在诸如 等革兰氏阴性菌中。在喀麦隆，虽然 已得到充分研究，但对于其他菌种如 、 和 却知之甚少。本研究评估了雅温得医院产碳青霉烯酶 的流行情况及相关危险因素。
采用来自四个医疗机构的531份临床样本进行了为期6个月的前瞻性横断面研究（2024年4月至9月）。通过形态学、生化和API 20 NE®试验鉴定菌株。使用纸片扩散法评估抗生素敏感性，并从表型上对碳青霉烯酶进行表征。
在样本中，4.9%分离出 ，其中 最为常见（80.8%），其次是 （11.5%）和 （7.7%）。 显示出较高的抗生素耐药性，尤其是对β-内酰胺类抗生素，85.71%的菌株产碳青霉烯酶（83.33%为B类，16.67%为A类）。显著的危险因素包括住院、当前抗生素治疗、近期抗生素使用以及38 - 47岁年龄组。
这些发现凸显了迫切需要加强监测、实施更严格的抗生素管理以及强化感染控制策略，以限制雅温得医疗机构中耐药 感染的传播。
