Yubolphan Ruedeemars, Phongsiri Kajhonpan, Mongkhammee Nanthakan, Pidech Jutamas, Roytrakul Sittiruk, Suwattanasophon Chonticha, Choowongkomon Kiattawee, Daduang Sakda, Khunkitti Watcharee, Jangpromma Nisachon
Department of Pharmacology, Faculty of Medicine Chiang Mai University Chiang Mai Thailand.
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI) Khon Kaen University Khon Kaen Thailand.
Food Sci Nutr. 2025 Aug 3;13(8):e70713. doi: 10.1002/fsn3.70713. eCollection 2025 Aug.
Egg white hydrolysate (EWH) has garnered significant attention for the therapeutic properties of their bioactive peptides. This study provides a comprehensive peptidomics analysis of EWH, focusing on its anti-inflammatory potential. Peptides were classified based on their anti-inflammatory potential (AIP) scores to identify promising candidates. In LPS-stimulated RAW 264.7 macrophages, specific peptides effectively suppressed nitric oxide (NO) production, a key inflammatory marker. IS8, PA11, and PK8 were identified as the most promising candidates. qRT-PCR analysis revealed their mechanism of action through modulation of key cytokines, including TNF-α, IL-1β, and IL-10, as well as other mediators such as iNOS and COX-2. Molecular docking studies provided additional insights into the anti-inflammatory potential of these peptides. Notably, IS8, PA11, and PK8 exhibited strong binding affinities to the iNOS active site, with IS8 and PK8 achieving the highest docking scores. The peptides engaged in critical interactions within the iNOS binding pocket through hydrogen bonding and π-π stacking with key residues, suggesting their potential to inhibit iNOS catalytic activity and thereby reduce NO production. These findings highlight the therapeutic potential of EWH-derived peptides, particularly IS8, PA11, and PK8, as anti-inflammatory agents through their ability to modulate key inflammatory pathways, with iNOS inhibition as a central mechanism.
蛋清水解物（EWH）因其生物活性肽的治疗特性而备受关注。本研究对EWH进行了全面的肽组学分析，重点关注其抗炎潜力。根据肽的抗炎潜力（AIP）得分对肽进行分类，以确定有前景的候选肽。在脂多糖刺激的RAW 264.7巨噬细胞中，特定的肽有效抑制了一氧化氮（NO）的产生，NO是一种关键的炎症标志物。IS8、PA11和PK8被确定为最有前景的候选肽。qRT-PCR分析揭示了它们通过调节关键细胞因子（包括TNF-α、IL-1β和IL-10）以及其他介质（如诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2））发挥作用的机制。分子对接研究为这些肽的抗炎潜力提供了更多见解。值得注意的是，IS8、PA11和PK8对iNOS活性位点表现出很强的结合亲和力，其中IS8和PK8获得了最高的对接分数。这些肽通过与关键残基形成氢键和π-π堆积在iNOS结合口袋内进行关键相互作用，表明它们具有抑制iNOS催化活性从而减少NO产生的潜力。这些发现突出了EWH衍生肽，特别是IS8、PA11和PK8作为抗炎剂的治疗潜力，它们能够通过调节关键炎症途径发挥作用，其中抑制iNOS是核心机制。
