Le Viet Hoang, King Tetyana, Wuerzberger Breanna, Bauer Olivia R, Carver Megan N, Chan Tiffany S, Henson Annabeth L, Hubbard Grace K, Kopadze Tamar, Patterson Claire F, McGraw Sabrina M, O'Hara Aidan, Yarkosky Eryk J, LaMontagne Michael G, Hotze Eileen M, Ferreira Rosana B R
Department of Molecular Biosciences, The University of Kansas, Lawrence, Kansas, USA.
Department of Biology and Biotechnology, University of Houston-Clear Lake, Houston, Texas, USA.
Microbiol Spectr. 2025 Aug 5:e0130625. doi: 10.1128/spectrum.01306-25.
The human skin microbiome is a diverse ecosystem that can help prevent infections by producing biomolecules and peptides that inhibit growth and virulence of bacterial pathogens. is a major human pathogen responsible for diseases that range from acute skin and soft tissue infections to life-threatening septicemia. Its ability to form biofilms is a key virulence factor contributing to its success as a pathogen as well as to its increased antimicrobial resistance. Here, we investigated the ability of bacterial skin commensals to produce molecules that inhibit biofilm formation. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified 77 human skin microbiome bacterial isolates from and genera. Metabolites from cell-free concentrated media (CFCM) from 26 representative isolates were evaluated for their ability to inhibit biofilm formation by both methicillin-resistant (MRSA) and methicillin-sensitive (MSSA) strains. CFCM, derived from most of the isolates, inhibited biofilm formation to varying extents but did not inhibit planktonic growth of . Size fractionation of the CFCM of three . isolates indicated that they produce different bioactive molecules. Cluster analysis, based on either MALDI-TOF mass spectra or whole-genome sequencing draft genomes, did not show clear clusters associated with levels of biofilm inhibition among strains. Finally, similar biosynthetic gene clusters were detected in all strains analyzed. These findings indicate that several bacterial constituents of the human skin microbiome display antibiofilm activity, warranting further investigation on their potential as novel therapeutic agents.
The skin is constantly exposed to the environment and consequently to numerous pathogens. The bacterial community that colonizes healthy skin is thought to play an important role in protecting us against infections. is a leading cause of death worldwide and is frequently involved in several types of infections, including skin and soft tissue infections. Its ability to adhere to surfaces and produce biofilms is considered an important virulence factor. Here, we analyzed the activity of different species of bacteria isolated from healthy skin on biofilm formation. We found that some species of and can reduce biofilm formation, although a generally lower level of inhibitory activity was observed compared to isolates. Among isolates, strength of activity was dependent on the strain. Our data highlight the importance of mining the skin microbiome for isolates that could help combat skin pathogens.
人类皮肤微生物群是一个多样化的生态系统，它可以通过产生抑制细菌病原体生长和毒力的生物分子和肽来帮助预防感染。金黄色葡萄球菌是一种主要的人类病原体，可导致从急性皮肤和软组织感染到危及生命的败血症等多种疾病。它形成生物膜的能力是其作为病原体成功以及抗微生物耐药性增加的关键毒力因素。在此，我们研究了皮肤共生细菌产生抑制金黄色葡萄球菌生物膜形成分子的能力。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）从葡萄球菌属和微球菌属中鉴定出77株人类皮肤微生物群细菌分离株。评估了来自26株代表性分离株的无细胞浓缩培养基（CFCM）的代谢产物抑制耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）菌株生物膜形成的能力。来自大多数分离株的CFCM在不同程度上抑制了生物膜形成，但未抑制金黄色葡萄球菌的浮游生长。对三株葡萄球菌分离株的CFCM进行大小分级表明它们产生不同的生物活性分子。基于MALDI-TOF质谱或全基因组测序草图基因组的聚类分析未显示与金黄色葡萄球菌菌株生物膜抑制水平相关的明显聚类。最后，在所有分析的金黄色葡萄球菌菌株中检测到相似的生物合成基因簇。这些发现表明，人类皮肤微生物群的几种细菌成分具有抗生物膜活性，值得进一步研究它们作为新型治疗剂的潜力。
皮肤不断暴露于环境中，因此会接触到众多病原体。定植于健康皮肤的细菌群落被认为在保护我们免受感染方面发挥着重要作用。金黄色葡萄球菌是全球主要的死亡原因，经常涉及多种类型的感染，包括皮肤和软组织感染。其粘附于表面并产生生物膜的能力被认为是一种重要的毒力因素。在此，我们分析了从健康皮肤分离的不同细菌物种对金黄色葡萄球菌生物膜形成的活性。我们发现，一些葡萄球菌属和微球菌属物种可以减少金黄色葡萄球菌生物膜形成，尽管与其他分离株相比，观察到的抑制活性水平普遍较低。在葡萄球菌分离株中，活性强度取决于菌株。我们的数据突出了挖掘皮肤微生物群中有助于对抗皮肤病原体的分离株的重要性。
