Yang Zhi, Lu Qian, Zheng Xucheng, Nazir Usman, Yang Haiming, Wang Zhiyue
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Poult Sci. 2025 Jul 23;104(10):105594. doi: 10.1016/j.psj.2025.105594.
This study evaluated the effects of varying dietary amylose/amylopectin ratios (AR) during starter (1-28 d) and grower (29-63 d) phases on growth performance, nutrient metabolism, and related molecular mechanisms in Jiangnan White geese. A total of 330 one-day-old male goslings were allocated to six groups in a 2 × 3 factorial design: two starter AR diets (0.34 or 0.46) and three grower AR diets (0.34, 0.46, or 0.58). The results showed that serum glucose increased post-prandially across all groups, with an increase at 120 min in the AR0.34/0.34 group (P < 0.05). Triglycerides (TG) decreased post-feeding in AR0.34/0.34, AR0.34/0.46, and AR0.46/0.46 groups (P < 0.05), while total cholesterol (TCHO) and high-density lipoprotein (HDL) increased in AR0.46/0.46 and AR0.46/0.58 groups (P < 0.05). Fasting insulin levels were elevated in AR0.34/0.46, AR0.34/0.58, and AR0.46/0.34 groups, whereas glucagon increased in AR0.46/0.46 and AR0.46/0.58 groups (P < 0.05). Liver glycogen content increased post-feeding in most groups, except AR0.34/0.34 and AR0.46/0.46. Pancreatic α-amylase activity decreased in AR0.46/0.58 at 120 min post-feeding, while jejunal α-amylase fluctuated temporally (P < 0.05). Gene expression analysis revealed dynamic regulation of glucose-6-phosphatase catalytic subunit 1 (G-6-PC-1), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS) postprandially, with higher fasting G-6-PC-1 and ACC expression in AR0.34/0.34 (P < 0.05). Results indicate that higher AR in the grower phase enhances postprandial glucose and lipid mobilization, modulates hormonal responses, and influences glycogen dynamics and starch-digesting enzyme activity. These findings suggest that amylose/amylopectin ratios differentially regulate glucose and lipid metabolism in geese, with implications for optimizing dietary formulations to improve nutrient utilization during distinct growth stages.
本研究评估了育雏期（1 - 28日龄）和生长期（29 - 63日龄）不同日粮直链淀粉/支链淀粉比例（AR）对江南白鹅生长性能、养分代谢及相关分子机制的影响。总共330只1日龄雄性雏鹅按2×3析因设计分为六组：两种育雏期AR日粮（0.34或0.46）和三种生长期AR日粮（0.34、0.46或0.58）。结果表明，所有组餐后血清葡萄糖均升高，AR0.34/0.34组在120分钟时升高（P < 0.05）。AR0.34/0.34、AR0.34/0.46和AR0.46/0.46组餐后甘油三酯（TG）降低（P < 0.05），而AR0.46/0.46和AR0.46/0.58组总胆固醇（TCHO）和高密度脂蛋白（HDL）升高（P < 0.05）。AR0.34/0.46、AR0.34/0.58和AR0.46/0.34组空腹胰岛素水平升高，而AR0.46/0.46和AR0.46/0.58组胰高血糖素升高（P < 0.05）。除AR0.34/0.34和AR0.46/0.46组外，大多数组餐后肝糖原含量增加。AR0.46/0.58组在餐后120分钟时胰腺α -淀粉酶活性降低，空肠α -淀粉酶随时间波动（P < 补0.05）。基因表达分析显示，餐后葡萄糖 - 6 -磷酸酶催化亚基1（G - 6 - PC - 1）、乙酰辅酶A羧化酶（ACC）、脂肪酸合酶（FAS）存在动态调控，AR0.34/0.34组空腹G - 6 - PC - 1和ACC表达较高（P < 0.05）。结果表明，生长期较高的AR可增强餐后葡萄糖和脂质动员，调节激素反应，并影响糖原动态和淀粉消化酶活性。这些发现表明，直链淀粉/支链淀粉比例对鹅的葡萄糖和脂质代谢有不同调节作用，这对优化日粮配方以提高不同生长阶段的养分利用率具有重要意义。
