豚鼠肾上腺异种生物代谢中的品系差异。

Strain differences in adrenal xenobiotic metabolism in guinea pigs.

作者信息

Colby H D, Rumbaugh R C, Marquess M L, Johnson P B, Pope M R, Stitzel R E

出版信息

Drug Metab Dispos. 1979 Sep-Oct;7(5):270-3.

Abstract

Studies were carried out to compare adrenal and hepatic xenobiotic metabolism in various strains of guinea pigs. In all strains studied (Hartley, English Short Hair, NIH, Strain 2, Strain 13), microsomal protein and cytochrome P-450 levels and NADPH-cytochrome c reductase activities were greater in adrenals than livers. Neither adrenal values nor hepatic values for these parameters differed across strains. Ethylmorphine (EM) demethylase and benzo[a]pyrene (BP) hydroxylase activities were also greater in adrenals than livers in all strains. However, the rates of adrenal xenobiotic metabolism were far greater in the highly inbred Strain 2 and Strain 13 guinea pigs than in other strains. In contrast, hepatic metabolism of EM and BP was not strain-dependent. Adrenal steroid 21-hydroxylase activity was also similar in all strains. The results indicate that strain is an important determinant of adrenal but not hepatic xenobiotic metabolism in the guinea pig. In addition, genetic control of adrenal microsomal drug and steroid metabolism appear to be independent of one another.

摘要

开展了多项研究以比较不同品系豚鼠的肾上腺和肝脏对外源生物的代谢情况。在所研究的所有品系（哈特利、英国短毛、美国国立卫生研究院品系、2号品系、13号品系）中，肾上腺中的微粒体蛋白、细胞色素P - 450水平以及NADPH - 细胞色素c还原酶活性均高于肝脏。这些参数的肾上腺值和肝脏值在不同品系间均无差异。在所有品系中，肾上腺中的N - 乙基吗啡（EM）脱甲基酶和苯并[a]芘（BP）羟化酶活性也高于肝脏。然而，在高度近交的2号品系和13号品系豚鼠中，肾上腺对外源生物的代谢速率远高于其他品系。相比之下，EM和BP的肝脏代谢不依赖于品系。肾上腺类固醇21 - 羟化酶活性在所有品系中也相似。结果表明，品系是豚鼠肾上腺对外源生物代谢的重要决定因素，但不是肝脏对外源生物代谢的重要决定因素。此外，肾上腺微粒体药物和类固醇代谢的遗传控制似乎相互独立。