Paik Youna, Kim You-Min, Choi Youn-Kyung, Kim Sung-Hun, Kim Seong-Sik, Kim Wooil, Kim Yong-Il
Department of Orthodontics, School of Dentistry, Dental Research Institute, Pusan National University, Geumoro 20, Mulgeum, Yangsan, 50612, Republic of Korea.
School of Dentistry, Institute of Dental and Life Sciences, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea.
Sci Rep. 2025 Aug 6;15(1):28664. doi: 10.1038/s41598-025-14004-0.
This study evaluated the mechanical and anti-bacterial properties of photocurable clear aligner resin (TC-85) incorporated with zwitterionic material, 2-methacryloyloxyethyl phosphorylcholine (MPC). Three experimental solutions were synthesized using 2-methacryloyloxyethyl phosphorylcholine (MPC) and clear aligner resin (TC-85). A group without any experimental solution served as a control. Specimens were fabricated by adding mixture of solutions to customized mold followed by post-curing procedure. Mechanical properties of the experimental groups were evaluated by tensile strength test, three-point flexural test, stress relaxation and creep test. Biological properties were evaluated through colony forming unit assay, MTT assay, and protein adsorption test. Incorporation of MPC into clear aligner resin achieved protein repellent and anti-microbial capabilities without compromising the mechanical properties. Tensile strength and three-point flexural test along with creep, stress relaxation test did not show significant difference in the mechanical properties. Addition of 1%, 2%, or 3% MPC into clear aligner resin significantly reduced the amount of bovine serum albumin adsorbed and proteins adsorbed from brain heart infusion medium by 1/3 ~ 1/4 that of a control (p < 0.001). The mixture of clear aligner resin with 3% MPC inhibited biofilm growth, reducing CFU counts by 40% compared to that of a control. Incorporating zwitterionic material with clear aligner resin TC-85 demonstrated an anti-biofouling activity while preserving its original mechanical properties, which may be a promising strategy to overcome the limitation of current clear aligner treatment.
本研究评估了掺入两性离子材料2-甲基丙烯酰氧乙基磷酰胆碱（MPC）的光固化透明矫治器树脂（TC-85）的机械性能和抗菌性能。使用2-甲基丙烯酰氧乙基磷酰胆碱（MPC）和透明矫治器树脂（TC-85）合成了三种实验溶液。一组不添加任何实验溶液作为对照。通过将溶液混合物添加到定制模具中，然后进行后固化程序来制备试样。通过拉伸强度测试、三点弯曲测试、应力松弛和蠕变测试评估实验组的机械性能。通过菌落形成单位测定、MTT测定和蛋白质吸附测试评估生物学性能。将MPC掺入透明矫治器树脂中可实现蛋白质排斥和抗菌能力，而不会损害机械性能。拉伸强度、三点弯曲测试以及蠕变、应力松弛测试在机械性能方面未显示出显著差异。向透明矫治器树脂中添加1%、2%或3%的MPC可使牛血清白蛋白吸附量以及从脑心浸液培养基中吸附的蛋白质量显著降低至对照的1/3至1/4（p<0.001）。含3%MPC的透明矫治器树脂混合物可抑制生物膜生长，与对照相比，菌落形成单位数量减少40%。将两性离子材料与透明矫治器树脂TC-85结合显示出抗生物污损活性，同时保留其原始机械性能，这可能是克服当前透明矫治器治疗局限性的一种有前景的策略。
