Zhang Ning, Melo Mary Anne S, Bai Yuxing, Xu Hockin H K
Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China; Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
J Dent. 2014 Oct;42(10):1284-91. doi: 10.1016/j.jdent.2014.07.016.
Biofilms at tooth-restoration margins can produce acids and cause secondary caries. A protein-repellent adhesive resin can potentially inhibit bacteria attachment and biofilm growth. However, there has been no report on protein-repellent dental resins. The objectives of this study were to develop a protein-repellent bonding agent incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC), and to investigate its resistance to protein adsorption and biofilm growth for the first time.
MPC was incorporated into Scotchbond Multi-Purpose (SBMP) at 0%, 3.75%, 7.5%, 11.25%, and 15% by mass. Extracted human teeth were used to measure dentine shear bond strengths. Protein adsorption onto resins was determined by a micro bicinchoninic acid (BCA) method. A dental plaque microcosm biofilm model with human saliva as inoculum was used to measure biofilm metabolic activity and colony-forming unit (CFU) counts.
Adding 7.5% MPC into primer and adhesive did not decrease the dentine bond strength, compared to control (p>0.1). Incorporation of 7.5% of MPC achieved the lowest protein adsorption, which was 20-fold less than that of control. Incorporation of 7.5% of MPC greatly reduced bacterial adhesion, yielding biofilm total microorganism, total streptococci, and mutans streptococci CFU that were an order of magnitude less than control.
A protein-repellent dental adhesive resin was developed for the first time. Incorporation of MPC into primer and adhesive at 7.5% by mass greatly reduced the protein adsorption and bacterial adhesion, without compromising the dentine bond strength.
The novel protein-repellent primer and adhesive are promising to inhibit biofilm formation and acid production, to protect the tooth-restoration margins and prevent secondary caries.
牙齿修复边缘的生物膜可产生酸并导致继发龋。一种抗蛋白质的粘结性树脂有可能抑制细菌附着和生物膜生长。然而，尚未有关于抗蛋白质牙科树脂的报道。本研究的目的是研发一种包含2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）的抗蛋白质粘结剂，并首次研究其对蛋白质吸附和生物膜生长的抗性。
按质量分数0%、3.75%、7.5%、11.25%和15%将MPC加入到Scotchbond多功能粘结剂（SBMP）中。使用拔除的人牙测量牙本质剪切粘结强度。通过微量双辛可宁酸（BCA）法测定树脂上的蛋白质吸附情况。以人唾液为接种物的牙菌斑微生态生物膜模型用于测量生物膜代谢活性和菌落形成单位（CFU）计数。
与对照组相比，在底漆和粘结剂中添加7.5%的MPC不会降低牙本质粘结强度（p>0.1）。加入7.5%的MPC实现了最低的蛋白质吸附，比对照组低20倍。加入7.5%的MPC大大降低了细菌附着力，使生物膜中的总微生物、总链球菌和变形链球菌CFU比对照组少一个数量级。
首次研发出一种抗蛋白质牙科粘结树脂。在底漆和粘结剂中按质量分数7.5%加入MPC可大大降低蛋白质吸附和细菌附着力，同时不影响牙本质粘结强度。
这种新型抗蛋白质底漆和粘结剂有望抑制生物膜形成和酸产生，保护牙齿修复边缘并预防继发龋。
