Ergün Yusuf, Baykişi Yaşarcan, Kılınç Metin, Mavigök Ayşe Nur, Mavigök Nadire, Toksözlü Furkan, Altıntaş Aykan Duygun
Department of Pharmacology, Kahramanmaraş Sütçü İmam University, Faculty of Medicine, Kahramanmaraş-Türkiye.
Department of Biochemistry, Kahramanmaraş Sütçü İmam University, Faculty of Medicine, Kahramanmaraş-Türkiye.
Ulus Travma Acil Cerrahi Derg. 2025 Aug;31(8):713-721. doi: 10.14744/tjtes.2025.52279.
Skeletal muscle ischemia-reperfusion injury (IRI) is a significant concern in various clinical settings. Oxidative stress and neutrophil infiltration play central roles in its pathophysiology. However, clinicians have very few therapeutic options for the prevention and treatment of IRI. The present study aimed to investigate the effects of epigallocatechin gallate (EGCG) on (i) skeletal muscle injury, (ii) oxidative stress, and (iii) markers related to neutrophil infiltration.
This was an experimental study conducted on rats. The IRI model involved the application of an elastic rubber band for 4 + 2 hours (IRI group). In the sham control (SC) group, all procedures were identical except for the rubber band application. Saline (10 mL/kg, intraperitoneally) and EGCG (25 or 50 mg/kg, intraperitoneally) were administered 30 minutes before reperfusion (IRI-SF, IRI-25, and IRI-50 groups, respectively). Creatine phosphokinase (CPK) was the primary endpoint. Other parameters included lactate dehydrogenase (LDH), total oxidant status (TOS), total antioxidant status (TAS), myeloperoxidase (MPO), E-selectin, P-selectin, L-selectin, intercellular adhesion molecule-1 (ICAM-1), and various cytokines (interleukin-1 beta [IL-1β], IL-6, and tumor necrosis factor-alpha [TNF-α]), which were measured in serum or gastrocnemius muscle samples.
CPK, LDH, and TOS levels were higher in the IRI group compared to the SC group (p=0.001, p=0.0001, and p=0.005, respectively). Although not statistically significant, decreases in these parameters were observed in the IRI-50 group compared to the IRI group (p=0.628, p=0.167, and p=0.444, respectively). Regarding TAS, a noticeable decrease was observed in the IRI group compared to the SC group (p=0.054), which was significantly increased by treatment with 50 mg/kg EGCG (p=0.011). For the remaining parameters (except IL-6), there were no statistically significant increases in the IRI group compared to SC, nor decreases in the EGCG-treated groups compared to the IRI group.
We propose that EGCG possesses antioxidant activity. However, any beneficial effect related to its interaction with neutrophil infiltration markers remains only suggestive.
骨骼肌缺血再灌注损伤（IRI）在各种临床情况下都是一个重要问题。氧化应激和中性粒细胞浸润在其病理生理学中起核心作用。然而，临床医生在预防和治疗IRI方面的治疗选择非常有限。本研究旨在探讨表没食子儿茶素没食子酸酯（EGCG）对（i）骨骼肌损伤、（ii）氧化应激和（iii）与中性粒细胞浸润相关标志物的影响。
这是一项在大鼠身上进行的实验研究。IRI模型包括应用弹性橡皮筋4 + 2小时（IRI组）。在假手术对照组（SC组）中，除了不应用橡皮筋外，所有操作均相同。在再灌注前30分钟分别腹腔注射生理盐水（10 mL/kg）和EGCG（25或50 mg/kg）（分别为IRI-SF、IRI-25和IRI-50组）。肌酸磷酸激酶（CPK）是主要终点。其他参数包括乳酸脱氢酶（LDH）、总氧化剂状态（TOS）、总抗氧化剂状态（TAS）、髓过氧化物酶（MPO）、E-选择素、P-选择素、L-选择素、细胞间黏附分子-1（ICAM-1）以及各种细胞因子（白细胞介素-1β[IL-1β]、IL-6和肿瘤坏死因子-α[TNF-α]），这些参数在血清或腓肠肌样本中进行测量。
与SC组相比，IRI组的CPK、LDH和TOS水平更高（分别为p = 0.001、p = 0.0001和p = 0.005）。尽管无统计学意义，但与IRI组相比，IRI-50组这些参数有所下降（分别为p = 0.628、p = 0.167和p = 0.444）。关于TAS，与SC组相比，IRI组明显下降（p = 0.054），用50 mg/kg EGCG治疗后显著升高（p = 0.011）。对于其余参数（IL-6除外），与SC组相比，IRI组无统计学意义的升高，与IRI组相比，EGCG治疗组也无下降。
我们认为EGCG具有抗氧化活性。然而，与其与中性粒细胞浸润标志物相互作用相关的任何有益作用仍只是推测性的。
