Asayama K, Sharp R A, Burr I M
Int J Biochem. 1985;17(11):1171-8. doi: 10.1016/0020-711x(85)90006-0.
Both murine cuprozinc and manganosuperoxide dismutases were purified to electrophoretic homogeneity from liver; the former (dimer) had a sp. act. of 2600 U/mg and a subunit mol. wt. of 17,000, the latter (tetramer) 2300 U/mg and mol. wt. 23,000. Heterologous radioimmunoassays had sensitivities of 3.1 ng/ml for the former enzyme and 2.5 ng/ml for the latter, and were appicable across murine genetic lines (Balb/c, ob/ob, db/db). Islets had the lowest concentrations of both enzymes among the tissues studied, and this could explain their vulnerability to free-radical damage.
小鼠的铜锌超氧化物歧化酶和锰超氧化物歧化酶均从肝脏中纯化至电泳纯；前者（二聚体）的比活性为2600 U/mg，亚基分子量为17,000，后者（四聚体）为2300 U/mg，分子量为23,000。异源放射免疫测定法对前者酶的灵敏度为3.1 ng/ml，对后者为2.5 ng/ml，且适用于不同的小鼠遗传品系（Balb/c、ob/ob、db/db）。在所研究的组织中，胰岛中这两种酶的浓度最低，这可以解释它们对自由基损伤的易感性。
