鸡肝中高分子量和低分子量酸性磷酸酶的分离及部分特性鉴定

Isolation and partial characterization of high and low molecular weight acid phosphatases from chicken liver.

作者信息

Panara F

出版信息

Int J Biochem. 1985;17(11):1213-7. doi: 10.1016/0020-711x(85)90011-4.

Abstract

Two acid phosphatase forms were isolated from chicken liver by gel filtration on Sephadex G-100. These enzymes, termed I and II, have similar Km- and Vmax-values, but differ in molecular weight, optimum pH, sensitivity to various inhibitors and substrate specificity. The results were compared with the numerous literature reports of mammalian acid phosphatases.

摘要

通过在葡聚糖凝胶G - 100上进行凝胶过滤，从鸡肝中分离出两种酸性磷酸酶形式。这些酶分别称为I和II，它们的米氏常数（Km）和最大反应速度（Vmax）值相似，但分子量、最适pH值、对各种抑制剂的敏感性和底物特异性不同。将这些结果与关于哺乳动物酸性磷酸酶的大量文献报道进行了比较。