Garnier D H
Gen Comp Endocrinol. 1985 Dec;60(3):414-8. doi: 10.1016/0016-6480(85)90075-9.
Sex steroid levels in the plasma of the female Pleurodeles waltl were determined by radioimmunoassay of blood samples taken monthly from each newt during an 18-month period. Estradiol-17 beta levels were relatively high (greater than 1 ng/ml) but androgen levels were higher (2-30 ng/ml). Androgen and estradiol-17 beta levels fluctuated according to season with two annual peaks, in autumn and in March. Testosterone was the principal androgen present in this plasma. Dihydrotestosterone levels were relatively low but showed slight fluctuations during the year. 4-Androstenedione and estrone levels were very low and relatively constant throughout the seasons. Fluctuations of androgen and estradiol-17 beta levels were synchronous between the several newts studied during the year. The ratio [E2-17 beta]/[Androgen] was stable during the major part of the year (0.2-0.3) except in July and August, when it reached the value of 1.0. The seasonal variations in the steroid levels were well correlated to the morphological modifications of sexual characteristics during the year.
在18个月的时间里，每月从雌性疣螈采集血样，通过放射免疫分析法测定其血浆中的性类固醇水平。雌二醇-17β水平相对较高（大于1纳克/毫升），但雄激素水平更高（2 - 30纳克/毫升）。雄激素和雌二醇-17β水平随季节波动，每年有两个峰值，分别在秋季和3月。睾酮是该血浆中主要的雄激素。双氢睾酮水平相对较低，但一年中略有波动。4-雄烯二酮和雌酮水平非常低，且全年相对稳定。在一年中，所研究的几只疣螈的雄激素和雌二醇-17β水平波动是同步的。除了7月和8月比值达到1.0外，一年中的大部分时间里[E2-17β]/[雄激素]的比值是稳定的（0.2 - 0.3）。类固醇水平的季节性变化与一年中性特征的形态学改变密切相关。
