Miranti Mia, Iskandar Iqbal Nur, Melanie Melanie, Malini Desak Made, Panatarani Camelia, Joni I Made, Prismantoro Dedat, Doni Febri, Joshi Ravindra Chandra, Hermawan Wawan
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, West Java, Indonesia; Functional Nano Powder University Centre of Excellence, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, West Java, Indonesia.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, West Java, Indonesia.
Virus Res. 2025 Aug 5;359:199614. doi: 10.1016/j.virusres.2025.199614.
Helicoverpa armigera nucleopolyhedrovirus (HearNPV1) shows considerable potential as a sustainable biological control agent against insect pests. Enhancing its efficacy through formulation with appropriate carrier materials is critical for improving pest management strategies. This study evaluated the potential of chitosan and zeolite nanoparticles as delivery vehicles for HearNPV1, assessing their effects on mortality and lethal time in second-instar Spodoptera litura larvae. A single-factor randomized block design with three replications was used. The treatments consisted of HearNPV1 formulated with various concentrations (0.125 % to 0.5 %) of either chitosan or zeolite nanoparticles. Larval mortality and lethal time (LT) were recorded and analyzed using one-way ANOVA, with significant differences further assessed by Duncan's multiple range test (α=0.05). Results showed that formulating HearNPV1 with either chitosan or zeolite nanoparticles significantly increased larval mortality. Notably, even the lowest concentration tested (0.125 %) of both chitosan and zeolite nanoparticles achieved 66.67 % larval mortality within seven days post-infection. However, no significant differences in lethal time were observed among treatments, regardless of nanoparticle type or concentration. Larvae exposed to virus-nanoparticle formulations exhibited lethal times ranging from 3.0 to 4.67 days on average-similar to the duration observed in larvae infected with HearNPV1 alone. In conclusion, both chitosan and zeolite nanoparticles at 0.125 % concentrations effectively enhanced HearNPV1-induced mortality in S. litura larvae, although they did not shorten the time to death. These findings underscore the potential for nanoparticle-based HearNPV1 formulations to improve biological pest control, warranting further investigation into their practical application and mechanisms of action.
棉铃虫核型多角体病毒（HearNPV1）作为一种可持续的害虫生物防治剂具有巨大潜力。通过与合适的载体材料配制来提高其效力对于改进害虫管理策略至关重要。本研究评估了壳聚糖和沸石纳米颗粒作为HearNPV1递送载体的潜力，评估它们对二龄斜纹夜蛾幼虫死亡率和致死时间的影响。采用单因素随机区组设计，重复三次。处理包括用不同浓度（0.125%至0.5%）的壳聚糖或沸石纳米颗粒配制的HearNPV1。记录幼虫死亡率和致死时间（LT），并使用单因素方差分析进行分析，通过邓肯多重范围检验（α = 0.05）进一步评估显著差异。结果表明，用壳聚糖或沸石纳米颗粒配制HearNPV1显著提高了幼虫死亡率。值得注意的是，即使壳聚糖和沸石纳米颗粒的最低测试浓度（0.125%）在感染后七天内也实现了66.67%的幼虫死亡率。然而，无论纳米颗粒类型或浓度如何，各处理之间在致死时间上均未观察到显著差异。暴露于病毒 - 纳米颗粒制剂的幼虫平均致死时间为3.0至4.67天，与仅感染HearNPV1的幼虫观察到的持续时间相似。总之，0.125%浓度的壳聚糖和沸石纳米颗粒均有效提高了HearNPV1诱导的斜纹夜蛾幼虫死亡率，尽管它们并未缩短死亡时间。这些发现强调了基于纳米颗粒的HearNPV1制剂在改善生物害虫控制方面的潜力，值得对其实际应用和作用机制进行进一步研究。
