Pu Zitian, Zhang Ruifang, Zhang Chi, Wang Hong, Wang Xin-Xin
State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Hebei, 071001, Baoding, People's Republic of China.
National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Taian, 271018, Shandong, People's Republic of China.
BMC Plant Biol. 2025 Aug 6;25(1):1031. doi: 10.1186/s12870-025-07023-6.
Changes in plant growth and root traits in wheat (Triticum aestivum L.) vary depending on the level of phosphorus (P) supply. Two important strategies for P acquisition in wheat are the release of carboxylates into the rhizosphere and the presence of arbuscular mycorrhizal fungi (AMF). However, the relationship between root exudates and P concentration in the shoot and root, as well as the role of AMF in this process, is not yet fully understood. This study was conducted utilizing three P supply rates (0, 50, and 200 mg P kg soil) in conjunction with AMF inoculation. We examined the effects of AMF on amount of rhizosphere carboxylates and plant P uptake for nine P contrasting wheat genotypes.
AMF decreased carboxylates, root biomass, root P content of wheat, and AMF reduced wheat root P allocation of wheat under all P levels. Notably, at 50 mg kg P level, the shoot P concentration of AMF-inoculated wheat exceeded that of other P levels, having a positive mycorrhizal responsiveness in all wheat genotypes. Furthermore, analysis revealed that wheat root morphology and acid phosphatase activity significantly influenced mycorrhizal growth responsiveness, while root carboxylates played a significant role in mycorrhizal P responsiveness.
The P acquisition of wheat was found to be contingent upon the interplay of root morphology, AMF, and carboxylate levels, with AMF and carboxylate playing a more crucial role in enhancing P absorption. Consequently, the current research provides important insights for nutrient management in wheat agricultural cultivation.
小麦（Triticum aestivum L.）的植物生长和根系性状变化取决于磷（P）供应水平。小麦获取磷的两个重要策略是向根际释放羧酸盐和丛枝菌根真菌（AMF）的存在。然而，根系分泌物与地上部和根部磷浓度之间的关系，以及AMF在此过程中的作用尚未完全了解。本研究利用三种磷供应速率（0、50和200毫克磷/千克土壤）并结合AMF接种进行。我们研究了AMF对九种磷差异小麦基因型根际羧酸盐量和植物磷吸收的影响。
AMF降低了小麦的羧酸盐、根生物量、根磷含量，并且在所有磷水平下，AMF都降低了小麦根的磷分配。值得注意的是，在50毫克/千克磷水平下，接种AMF的小麦地上部磷浓度超过了其他磷水平，在所有小麦基因型中都具有正的菌根响应性。此外，分析表明，小麦根系形态和酸性磷酸酶活性显著影响菌根生长响应性，而根系羧酸盐在菌根磷响应中起重要作用。
发现小麦的磷获取取决于根系形态、AMF和羧酸盐水平的相互作用，其中AMF和羧酸盐在增强磷吸收方面发挥更关键的作用。因此，本研究为小麦农业种植中的养分管理提供了重要见解。
