Zhao Chenxi, Zhan Minmin, Du Hengjun, Zhu Kai, Chen Shiguo, Song Mingyue, Cao Yong, Xiao Hang
Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, United States.
J Agric Food Chem. 2025 Aug 20;73(33):20888-20899. doi: 10.1021/acs.jafc.5c08064. Epub 2025 Aug 6.
Pectin, as an important component of dietary fiber, plays a crucial regulatory role in maintaining the homeostasis of gut microbiota. The transient alternate high-fat diet (TAHD) is a dietary pattern that alternates between 3 days of a low-fat diet (LFD) and 3 days of a high-fat diet, aiming to simulate daily dietary habits. In this study, we investigated the temporal dynamics of gut microbiota and short-chain fatty acids (SCFAs) in response to citrus pectin (CP, 3% (w/w)) supplementation under TAHD. CP effectively reduced the abundance of the phylum Bacillota and promoted the phylum Bacteroidota. During the LFD phase, CP significantly increased colonization abundance (3.76-fold within 1 day; < 0.05) and promoted SCFA biosynthesis. In addition, the synergistic intervention of CP enhanced the thermogenic metabolism of the host by activating the mRNA expression levels of thermogenesis-related genes (such as UCP1, etc.) in brown adipose tissue. This series of studies systematically elucidated the molecular mechanism by which CP improves host metabolic homeostasis by optimizing the structure of the gut microbiota from the perspective of the microbiome-metabolism axis, providing an important theoretical basis for the application of dietary fiber in the prevention and treatment of metabolic diseases.
果胶作为膳食纤维的重要组成部分，在维持肠道微生物群稳态方面发挥着关键的调节作用。短期交替高脂饮食（TAHD）是一种在低脂饮食（LFD）3天和高脂饮食3天之间交替的饮食模式，旨在模拟日常饮食习惯。在本研究中，我们研究了在TAHD条件下补充柑橘果胶（CP，3%（w/w））后肠道微生物群和短链脂肪酸（SCFAs）的时间动态变化。CP有效降低了厚壁菌门的丰度，并促进了拟杆菌门的生长。在LFD阶段，CP显著增加了定植丰度（1天内增加3.76倍；P<0.05）并促进了SCFA的生物合成。此外，CP的协同干预通过激活棕色脂肪组织中产热相关基因（如UCP1等）的mRNA表达水平，增强了宿主的产热代谢。这一系列研究从微生物组-代谢轴的角度系统地阐明了CP通过优化肠道微生物群结构改善宿主代谢稳态的分子机制，为膳食纤维在代谢性疾病防治中的应用提供了重要的理论依据。
