Postbiotics enhance the efficacy of derivative compound mouthwash against clinical strains.

BACKGROUND

A previous study indicated that poly L-lysine-glycerol monolaurate mouthwash reduced the virulence of ; however, these compounds are derivatives. Thus, this study aimed to compare the effects of postbiotics, postbiotic-glycerol monolaurate, and poly L-lysine-glycerol monolaurate mouthwashes against clinical strains.

METHODS

Postbiotics, SD1, SD4, and SD11 were examined for anti-bacterial activity and synergistic effects. Subsequently, mouthwashes containing postbiotics, postbiotic-glycerol monolaurate, and poly L-Lysine-glycerol monolaurate were prepared and evaluated for their ability to reduce adhesion to host cells, suppress inflammation induced by , eradicate biofilm, decrease A expression, and assess epithelial cell viability. The stability of the mouthwashes was evaluated every 4 weeks up to 24 weeks for their efficacy against growth, biofilm eradication, and epithelial cell viability.

RESULTS

The postbiotics, SD1 and SD11, demonstrated significant anti- activity, with synergistic effects observed in combinations with derivative compounds. Postbiotic-glycerol monolaurate mouthwashes exhibited higher efficacy in reducing adhesion to host cells (42.64-43.83%), suppressing pro-inflammatory cytokines, eradicating biofilm (82.62% at 24 h), and reducing A expression (112.60 fold) compared to others. Such mouthwashes also displayed low cytotoxicity (< 30% for 15 min) to all cells tested. The stability was observed up to 24 weeks.

CONCLUSION

This study demonstrated that postbiotic-glycerol monolaurate mouthwash revealed the highest efficacy against with low cytotoxicity to host cells. The stability lasted for 24 weeks.