Juntarachot Nucharee, Sunpaweravong Somkiat, Kaewdech Apichat, Wongsuwanlert Mutita, Ruangsri Praphansri, Pahumunto Nuntiya, Teanpaisan Rawee
Research Center of Excellence for Oral Health, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Thailand.
Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
J Taibah Univ Med Sci. 2023 Mar 10;18(5):1048-1054. doi: 10.1016/j.jtumed.2023.02.017. eCollection 2023 Oct.
To characterize the adhesion ability of nine strains and eight probiotics in human oral keratinocyte cells (H357 cells) in comparison to intestinal cells (Caco-2 and HIEC-6 cells). Subsequently, the anti-adhesion and co-aggregation abilities of the selected probiotic strains on strains were investigated.
Nine strains, including ATCC43504 (type strain), and 8 clinical strains, were isolated from oral samples of three patients (one non-disease, one gastritis patient, and one gastric cancer patient). Eight selected probiotic strains were used, as follows: SD1, SD4 SD11, SD7, GG, ATCC-PTA6475, Shirota, and CNCM I-1572. The adhesion and anti-adhesion abilities of and the probiotic strains were investigated in H357, Caco-2, and HIEC-6 cells. Co-aggregation at various pHs, hydrophobicity, and surface receptors of the cell lines for strains were examined.
All probiotic and strains adhered to H357 significantly better than Caco-2, and HIEC-6 cells. Three probiotic strains (SD7, SD4, SD11) showed significantly higher adhesion than others. Of the clinical strains, isolates from a gastric cancer patient had the highest adhesion ability to all of the cell lines tested. Probiotic strains that exhibited high adhesion ability provided high anti-adhesion and co-aggregation against strains. Acidic conditions encouraged the co-aggregation of probiotics to strains.
This study provides information relating to the adhesion abilities of clinical and probiotic strains to the oral mucosa when compared to the intestinal mucosa. Certain probiotic strains may be useful for the successful eradication of infection anti-adhesion and co-aggregation.
与肠道细胞（Caco - 2和HIEC - 6细胞）相比，表征9株幽门螺杆菌和8株益生菌在人口腔角质形成细胞（H357细胞）中的黏附能力。随后，研究所选益生菌菌株对幽门螺杆菌的抗黏附和共聚集能力。
从三名患者（一名非疾病患者、一名胃炎患者和一名胃癌患者）的口腔样本中分离出9株幽门螺杆菌，包括幽门螺杆菌ATCC43504（模式菌株）和8株临床菌株。使用8株所选益生菌菌株，如下：嗜酸乳杆菌SD1、嗜酸乳杆菌SD4、嗜酸乳杆菌SD11、嗜酸乳杆菌SD7、鼠李糖乳杆菌GG、嗜酸乳杆菌ATCC - PTA6475、干酪乳杆菌Shirota和植物乳杆菌CNCM I - 1572。在H357、Caco - 2和HIEC - 6细胞中研究幽门螺杆菌和益生菌菌株的黏附和抗黏附能力。检测了不同pH值下幽门螺杆菌菌株的共聚集、疏水性以及细胞系的表面受体。
所有益生菌和幽门螺杆菌菌株在H357细胞上的黏附明显优于Caco - 2和HIEC - 6细胞。三株益生菌菌株（SD7、SD4、SD11）的黏附明显高于其他菌株。在临床幽门螺杆菌菌株中，来自胃癌患者的分离株对所有测试细胞系的黏附能力最高。表现出高黏附能力的益生菌菌株对幽门螺杆菌菌株具有高抗黏附和共聚集能力。酸性条件促进了益生菌与幽门螺杆菌菌株的共聚集。
本研究提供了与临床幽门螺杆菌和益生菌菌株与肠道黏膜相比在口腔黏膜上的黏附能力相关的信息。某些益生菌菌株可能有助于通过抗黏附和共聚集成功根除幽门螺杆菌感染。
