Becerra Daniela, Vargas-Torres Valentina, Veloso-Giménez Valentina, Gallardo-Agüero Daniela, Miranda Miguel, Hernández-Pavez Valentina, González-Quezada Nicolás, San Martín Sebastián, Boric Mauricio P, Egaña José Tomás
Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, Santiago 7820436, Chile.
Center of Interdisciplinary Biomedical and Engineering Research for Health (MEDING), School of Medicine, Universidad de Valparaíso, Angamos 655, Viña del Mar 2540064, Chile.
ACS Appl Bio Mater. 2025 Aug 18;8(8):7433-7448. doi: 10.1021/acsabm.5c01137. Epub 2025 Aug 7.
The delivery of photosynthetic microorganisms has emerged as a strategy for tissue oxygenation, offering a promising approach to treat several hypoxic conditions. Among these, intravascular photosynthesis has been proposed for organ preservation; however, the most suitable photosynthetic microorganisms and their behavior during intravascular perfusion remain to be fully elucidated. Therefore, this study evaluates key properties of photosynthetic solutions for organ perfusion, based on the microalgae and the cyanobacterium . characterization showed that both microorganisms maintained viability, morphology, and oxygen production capacity in a Ringer's lactate-based medium for at least 24 h, with both photosynthetic solutions exhibiting rheological properties compatible with organ perfusion. perfusion of rat kidneys demonstrates sustained hemodynamic stability, with showing lower variability in vascular resistance. Histological analysis revealed significant retention of both microorganisms within renal structures, with inducing less tubular damage. Additionally, biocompatibility assays with human endothelial cells and zebrafish larvae showed no significant cytotoxic effects of the photosynthetic solutions. These findings support the feasibility of using photosynthetic microorganisms for intravascular photosynthesis, highlighting as particularly promising due to its lower oxygen consumption in darkness and reduced tissue damage after perfusion. This work provides significant insights toward the development of biologically active perfusion systems for innovative preservation strategies for organ transplantation.
光合微生物的递送已成为一种组织氧合策略，为治疗多种缺氧状况提供了一种有前景的方法。其中，血管内光合作用已被提议用于器官保存；然而，最合适的光合微生物及其在血管内灌注过程中的行为仍有待充分阐明。因此，本研究基于微藻和蓝细菌评估了用于器官灌注的光合溶液的关键特性。表征显示，两种微生物在基于乳酸林格氏液的培养基中至少24小时内保持活力、形态和产氧能力，两种光合溶液均表现出与器官灌注相容的流变学特性。大鼠肾脏灌注显示出持续的血流动力学稳定性，[此处原文缺失具体比较内容]显示血管阻力变异性较低。组织学分析显示两种微生物在肾脏结构内均有显著滞留，[此处原文缺失具体比较内容]导致的肾小管损伤较少。此外，对人内皮细胞和斑马鱼幼虫的生物相容性检测表明光合溶液没有显著的细胞毒性作用。这些发现支持了使用光合微生物进行血管内光合作用的可行性，[此处原文缺失具体比较内容]由于其在黑暗中耗氧量较低且灌注后组织损伤减少而显得特别有前景。这项工作为开发用于器官移植创新保存策略的生物活性灌注系统提供了重要见解。
