Werner Jonathan M, Suresh Hamsini, Gillis Jesse
Physiology Department and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
bioRxiv. 2025 Jul 24:2025.07.23.666342. doi: 10.1101/2025.07.23.666342.
Subclass cell-types are strongly conserved across mammalian brains, making the systemic absence of a caudal ganglionic eminence (CGE) Pax6 interneuron subclass in mouse transcriptional atlases unexpected. Through cross-species transcriptomic analysis, we identify a Pax6 subclass homolog in mouse and uncover primate-specific divergence in the Sncg subclass. Our results highlight the pitfalls of relying on single marker genes and provide evolution-aware annotations and marker sets to support robust cross-mammal interneuron comparisons.
亚类细胞类型在整个哺乳动物大脑中高度保守，这使得小鼠转录图谱中尾侧神经节隆起（CGE）Pax6中间神经元亚类的系统性缺失出乎意料。通过跨物种转录组分析，我们在小鼠中鉴定出一个Pax6亚类同源物，并发现Sncg亚类中存在灵长类特异性差异。我们的结果突出了依赖单一标记基因的缺陷，并提供了具有进化意识的注释和标记集，以支持可靠的跨哺乳动物中间神经元比较。
