Wang Jixia, Wang Mingxiao, Wang Yuting, Xu Mengbo, Liu Yang, Zheng Mingxue, Zhao Rui, Bai Rui, Zhao Yanping, Zhang Li, Lv Xiaoling, Yang Yu, Guan Wenchao, Cui Xiaozhen
College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China.
College of Animal Science, Shanxi Agricultural University, Jinzhong, China.
Front Vet Sci. 2025 Jul 24;12:1595384. doi: 10.3389/fvets.2025.1595384. eCollection 2025.
Annexin A2 (ANXA2) is a multifunctional protein involved in host-pathogen interactions during viral and parasitic infections. To investigate the role of ANXA2 in host cell apoptosis induced by , RNA interference (RNAi) was employed to specifically downregulate ANXA2 expression. Primary cultures of chicken embryonic cecal epithelial cells were established and subjected to sporozoite infection. A comprehensive analytical approach integrating hematoxylin-eosin staining, Hoechst-Annexin V-PI triple-staining, and caspase-3 activity quantification was used. Western-blot and RT-qPCR were performed to assess transcriptional and translational changes in key apoptosis-related factors, including B-cell lymphoma (Bcl-2) and Bcl-2-associated X protein (Bax). Additionally, the dynamic expression of ANXA2 was analyzed to clarify its function in the parasite-host interaction. The results showed that the ANXA2 expression in the group increased at 4 h after inoculation but decreased at 24 to 96 h compared to the control group ( < 0.01). Following ANXA2 knockdown, the cell apoptosis rate, caspase-3 activity, and Bax expression levels were significantly increased ( < 0.01), whereas the infection rate and Bcl-2 expression levels were significantly decreased ( < 0.01) compared to the group infected with alone. In conclusion, ANXA2 serves as a critical regulator of host cell responses during infection. RNAi-mediated suppression of ANXA2 expression significantly enhances apoptosis induced by . This study establishes a foundation for further exploration of therapeutic targets to reduce host tissue damage, indicating that targeting ANXA2 may be a viable approach for controlling coccidiosis.
膜联蛋白A2（ANXA2）是一种多功能蛋白，参与病毒和寄生虫感染期间的宿主-病原体相互作用。为了研究ANXA2在由[未提及具体病原体名称]诱导的宿主细胞凋亡中的作用，采用RNA干扰（RNAi）特异性下调ANXA2表达。建立鸡胚盲肠上皮细胞原代培养物，并使其受到[未提及具体病原体名称]子孢子感染。采用了综合分析方法，包括苏木精-伊红染色、Hoechst-膜联蛋白V-碘化丙啶三重染色以及半胱天冬酶-3活性定量分析。进行蛋白质免疫印迹（Western-blot）和逆转录定量聚合酶链反应（RT-qPCR）以评估关键凋亡相关因子的转录和翻译变化，这些因子包括B细胞淋巴瘤（Bcl-2）和Bcl-2相关X蛋白（Bax）。此外，分析了ANXA2的动态表达以阐明其在寄生虫-宿主相互作用中的功能。结果显示，与对照组相比，[未提及具体病原体名称]组接种后4小时ANXA2表达增加，但在24至96小时降低（P<0.01）。与仅感染[未提及具体病原体名称]的组相比，敲低ANXA2后，细胞凋亡率、半胱天冬酶-3活性和Bax表达水平显著增加（P<0.01），而感染率和Bcl-2表达水平显著降低（P<0.01）。总之，ANXA2在[未提及具体病原体名称]感染期间作为宿主细胞反应的关键调节因子。RNAi介导的ANXA2表达抑制显著增强了由[未提及具体病原体名称]诱导的细胞凋亡。本研究为进一步探索减少宿主组织损伤的治疗靶点奠定了基础，表明靶向ANXA2可能是控制球虫病的一种可行方法。
