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对白血病单核细胞系抗炎活性的体外科学验证

In-vitro scientific validation of anti-inflammatory activity of on Leukemia monocytic cell line.

作者信息

Dutta Sharmistha, Nishad Khushbu, Usha Talambedu, Ramesh Nijalingappa, Middha Sushil Kumar

机构信息

Department of Biotechnology, School of Applied Sciences, REVA university, Rukmini Knowledge Park, Bengaluru-560064, India.

Department of Biochemistry, Maharani Lakshmi Ammanni College For Women, Bengaluru-560 012, India.

出版信息

Afr Health Sci. 2024 Sep;24(3):128-137. doi: 10.4314/ahs.v24i3.17.

DOI:10.4314/ahs.v24i3.17
PMID:40777945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12327121/
Abstract

BACKGROUND

The induction of the inflammatory cascade results in the production of a number of inflammatory mediators, including prostaglandin E2 (PGE2), nitric oxide (NO), and proinflammatory cytokines like TNF-, IL-, and IL-6. This study examined the cytotoxicity and anti-inflammatory properties of a methanolic crude extract of Punica granatum (PPM) on monocytic leukaemia cell line (THP-1).

MATERIALS AND METHODS

The PPM along with Quercetin as reference was used to assess the cytotoxic effect on THP-1 cells and describe its effect on pro-inflammatory cytokines such as COX-2, TNF-α, IL-6 against cancer cell line by flow cytometry.

RESULTS

The percentage of viable cells significantly decreased which correlates to non-toxicity whereas quercetin was found to be highly toxic, the IC50 could not be calculated because of drug precipitation. There was a significant decrease in the expressions of inflammatory cytokines upon pre-treatment of the cells with PPM prior to LPS stimulation.

CONCLUSION

Our findings indicate that no cytotoxicity was observed after the treatment of THP-1 cells with PPM (25-400 µg/ml), but at higher concentration (400µg/ml), the cell viability decreased to 84% and attenuated the expression level of inflammatory cytokines. The inhibitory effect of the extract on pro-inflammatory factors production may provide a theoretical source on upcoming treatment of inflammation.

摘要

背景

炎症级联反应的诱导会导致多种炎症介质的产生，包括前列腺素E2（PGE2）、一氧化氮（NO）以及促炎细胞因子，如肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和白细胞介素-6（IL-6）。本研究检测了石榴甲醇粗提物（PPM）对单核细胞白血病细胞系（THP-1）的细胞毒性和抗炎特性。

材料与方法

使用PPM以及作为对照的槲皮素来评估对THP-1细胞的细胞毒性作用，并通过流式细胞术描述其对癌细胞系中诸如环氧化酶-2（COX-2）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）等促炎细胞因子的影响。

结果

活细胞百分比显著下降，这与无毒性相关，而槲皮素被发现具有高毒性，由于药物沉淀无法计算半数抑制浓度（IC50）。在用脂多糖（LPS）刺激之前用PPM预处理细胞后，炎症细胞因子的表达显著降低。

结论

我们的研究结果表明，用PPM（25 - 400微克/毫升）处理THP-1细胞后未观察到细胞毒性，但在较高浓度（400微克/毫升）下，细胞活力降至84%，并减弱了炎症细胞因子的表达水平。该提取物对促炎因子产生的抑制作用可能为未来炎症治疗提供理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/637bae0651e1/AFHS2403-0128Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/15d18b797d35/AFHS2403-0128Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/9808bf602c52/AFHS2403-0128Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/643bbc2c16f2/AFHS2403-0128Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/f84c7b4de8e1/AFHS2403-0128Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/637bae0651e1/AFHS2403-0128Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/15d18b797d35/AFHS2403-0128Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/9808bf602c52/AFHS2403-0128Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/643bbc2c16f2/AFHS2403-0128Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/f84c7b4de8e1/AFHS2403-0128Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0dc/12327121/637bae0651e1/AFHS2403-0128Fig5.jpg

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