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一种从肌球蛋白制备亚片段1的简化方法。

A streamlined method of subfragment one preparation from myosin.

作者信息

Okamoto Y, Sekine T

出版信息

J Biochem. 1985 Oct;98(4):1143-5. doi: 10.1093/oxfordjournals.jbchem.a135365.

DOI:10.1093/oxfordjournals.jbchem.a135365
PMID:4077843
Abstract

A rapid procedure for isolating subfragment one (SF1) from myosin was found. SF1 can be isolated specifically from proteolytic digests of myosin in the presence of a millimolar concentration of magnesium chloride. Under such ionic conditions all of the rod portion and undigested myosin is selectively precipitated. A nucleotide trapping experiment indicated how important quick preparation of SF1 is for maintaining the active site structure. This method can also be utilized in the preparation of heavy meromyosin.

摘要

发现了一种从肌球蛋白中分离亚片段一（SF1）的快速方法。在毫摩尔浓度的氯化镁存在下，可从肌球蛋白的蛋白水解消化物中特异性分离出SF1。在这种离子条件下，所有的杆状部分和未消化的肌球蛋白会被选择性沉淀。一项核苷酸捕获实验表明，快速制备SF1对于维持活性位点结构有多重要。该方法也可用于制备重酶解肌球蛋白。

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A streamlined method of subfragment one preparation from myosin.一种从肌球蛋白制备亚片段1的简化方法。
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