Takeuchi R, Imanaka T, Ohkuma S, Takano T
J Biochem. 1985 Oct;98(4):933-8. doi: 10.1093/oxfordjournals.jbchem.a135373.
Cholesterol oleate liquid crystals were prepared in vitro as a model of lipid droplets accumulated in the atheromatous aorta. The hydrolysis of cholesterol oleate crystals by lysosomal acid lipase was examined in the presence and absence of various phospholipids. Phosphatidylserine markedly stimulated the hydrolysis of cholesterol oleate liquid crystals (20 times the basal value); it increased the Vmax value about 15 times and decreased the Km value to 1/20 times the basal value. The polar head group and the acyl chains of phosphatidylserine were required for its stimulation of hydrolysis.
体外制备了胆固醇油酸液晶，作为动脉粥样硬化主动脉中积累的脂滴模型。在存在和不存在各种磷脂的情况下，研究了溶酶体酸性脂肪酶对胆固醇油酸晶体的水解作用。磷脂酰丝氨酸显著刺激胆固醇油酸液晶的水解（是基础值的20倍）；它使Vmax值增加约15倍，并使Km值降至基础值的1/20。磷脂酰丝氨酸的极性头部基团和酰基链对其刺激水解是必需的。
