Vieira Caroline Krug, Dos Anjos Borges Luiz Gustavo, Marascalchi Matheus Nicoletti, Russi Carlos Henrique, Marandola Tamiris, Kemmelmeier Karl, Soares Cláudio Roberto Fonsêca Sousa, Stürmer Sidney Luiz, Giongo Adriana
Universidade Regional de Blumenau (FURB), Programa de Pós-Graduação em Engenharia Ambiental, Blumenau, Brazil.
Laboratory of Fungal Biology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic.
Mycorrhiza. 2025 Aug 8;35(4):49. doi: 10.1007/s00572-025-01218-3.
The recovery of the soil ecosystem after severe disturbances, such as coal-mining activities, depends on both abiotic and biotic improvements. This study assessed the influence of arbuscular mycorrhizal (AM) fungal consortia on microbial community dynamics across two stages of soil recovery - 2 years (2Y) and 15 years (15Y) post-disturbance - using a secondary succession forest (SSR) as a reference. We analyzed bacterial community composition via 16 S rRNA gene amplicon sequencing and evaluated key soil quality indicators. While inoculation with AM fungal consortia had minimal effects on most soil parameters, significant differences were observed between recovery stages. The 15Y recovery site exhibited improved soil structure, microbial activity, and aggregate stability compared to the 2Y site, highlighting the importance of long-term restoration. However, potential overlap in ecological roles among native microorganisms likely mitigates the impact of AMF inoculation. These findings suggest that AM fungal consortia alone may not drive immediate improvements in soil quality but can contribute to microbial interactions and recovery processes over time. This study highlights the complexity of soil restoration and emphasizes the need for strategies that integrate plant cover with microbial community development to enhance long-term ecosystem stability. Further research should explore the specific roles of AM fungi and native soil microbes in promoting soil structure and accelerating recovery.
在诸如煤矿开采活动等严重干扰之后，土壤生态系统的恢复取决于非生物和生物方面的改善。本研究以次生演替森林（SSR）作为参照，评估了丛枝菌根（AM）真菌群落对土壤恢复两个阶段（干扰后2年（2Y）和15年（15Y））微生物群落动态的影响。我们通过16S rRNA基因扩增子测序分析了细菌群落组成，并评估了关键土壤质量指标。虽然接种AM真菌群落对大多数土壤参数的影响极小，但在恢复阶段之间观察到了显著差异。与2Y站点相比，15Y恢复站点的土壤结构、微生物活性和团聚体稳定性有所改善，突出了长期恢复的重要性。然而，本地微生物之间生态作用的潜在重叠可能减轻了AMF接种的影响。这些发现表明，单独的AM真菌群落可能不会立即推动土壤质量的改善，但随着时间的推移可以促进微生物相互作用和恢复过程。本研究突出了土壤恢复的复杂性，并强调需要采取将植物覆盖与微生物群落发展相结合的策略，以增强长期生态系统稳定性。进一步的研究应探索AM真菌和本地土壤微生物在促进土壤结构和加速恢复方面的具体作用。
