Qiu Wei, Kang Jie, Ye Zeming, Yang Shengdie, Tu Xiujun, Xie Penghao, Ge Jingping, Ping Wenxiang, Yuan Jun
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, 150080, China.
Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
New Phytol. 2025 May;246(3):1276-1292. doi: 10.1111/nph.70064. Epub 2025 Mar 19.
The assembly of the rhizosphere microbiome determines its functionality for plant fitness. Although the interactions between arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) play important roles in plant growth and disease resistance, research on the division of labor among the members of the symbionts formed among plants, AMF, and PGPR, as well as the flow of carbon sources, is still insufficient. To address the above questions, we used soybean (Glycine max), Funneliformis mosseae, and Pseudomonas putida KT2440 as research subjects to establish rhizobiont interactions and to elucidate the signal exchange and division of labor among these components. Funneliformis mosseae can attract P. putida KT2440 by secreting cysteine as a signaling molecule and can promote the colonization of P. putida KT2440 in the soybean rhizosphere. Colonized P. putida KT2440 can stimulate the l-tryptophan secretion of the host plant and can lead to the upregulation of genes involved in converting methyl-indole-3-acetic acid (Me-IAA) into IAA in response to l-tryptophan stimulation. Collectively, we decipher the tripartite mechanism of rhizosphere microbial community assembly via cross-kingdom interactions.
根际微生物群的组装决定了其对植物健康的功能。尽管丛枝菌根真菌（AMF）与促植物生长根际细菌（PGPR）之间的相互作用在植物生长和抗病性中发挥着重要作用，但关于植物、AMF和PGPR之间形成的共生体成员之间的分工以及碳源流动的研究仍然不足。为了解决上述问题，我们以大豆（Glycine max）、摩西管柄囊霉（Funneliformis mosseae）和恶臭假单胞菌KT2440（Pseudomonas putida KT2440）为研究对象，建立根际共生体相互作用，阐明这些组分之间的信号交换和分工。摩西管柄囊霉可以通过分泌半胱氨酸作为信号分子来吸引恶臭假单胞菌KT2440，并可以促进恶臭假单胞菌KT2440在大豆根际的定殖。定殖后的恶臭假单胞菌KT2440可以刺激宿主植物分泌L-色氨酸，并可以导致响应L-色氨酸刺激将甲基吲哚-3-乙酸（Me-IAA）转化为IAA的相关基因上调。总的来说，我们通过跨界相互作用破译了根际微生物群落组装的三方机制。
