精子唾液酸酶NEU1和NEU3是体外受精受精率的潜在预测指标：一项前瞻性队列研究。

Spermatozoal sialidases NEU1 and NEU3 are potential predictors for fertilization rate in IVF: a prospective cohort study.

作者信息

Yuan Xin, Li Jiajie, Deng Tang, Yang Li, Wu Yilun, Xiang Liangchen, Yuan Fang, Sun Xinrui, Xia Yi, Yang Yihong, Zhang Yi, Ma Fang, Wang Yan

机构信息

Center for Translational Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.

Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.

出版信息

J Assist Reprod Genet. 2025 Aug 8. doi: 10.1007/s10815-025-03602-6.

DOI:10.1007/s10815-025-03602-6

PMID:40779284

Abstract

PURPOSE

This study aimed to explore the roles of spermatozoal sialidase NEU1 and NEU3 in affecting the fertilization rate of in vitro fertilization (IVF), and whether sperm sialidase NEU1 and NEU3 can be used as predictors of IVF outcome.

MATERIAL AND METHODS

This was a prospective cohort study that collected semen samples from 194 IVF couples between January 2024 and April 2024. Patients were grouped based on oocyte maturity and routine semen analysis. Detection of sperm NEU1 and NEU3 was carried out in semen from the same ejaculate for IVF by using flow cytometry, followed by correlation analysis with fertilization rates.

RESULTS

NEU1 and NEU3 are independent indicators separate from regular semen parameters. With a metaphase II (MII) rate ≥80% and normal semen routine analysis, sperm NEU1 (P = 0.0300) and NEU3 (P = 0.0253) levels were significantly different demarcated by 80% fertilization rate. Lower NEU1 and NEU3 expression on sperm is associated with lower fertilization rates. ROC curve analyses displaying the diagnostic accuracy of NEU1 (P = 0.0306, AUC = 0.660) and NEU3 (P = 0.0259, AUC = 0.665). χ test analyzes the fertilization rate and the different cut-off rate for NEU1 (χ = 4.671, P = 0.0307) or NEU3 (χ = 6.377, P = 0.0116).

CONCLUSIONS

NEU1 and NEU3 on sperm can predict the IVF fertilization outcome in the group with oocyte MII rate ≥80%. The protein levels of NEU1 and NEU3 on sperm could be a new index for assisted reproductive technology (ART).

摘要

目的

本研究旨在探讨精子唾液酸酶NEU1和NEU3在影响体外受精（IVF）受精率方面的作用，以及精子唾液酸酶NEU1和NEU3是否可作为IVF结局的预测指标。

材料与方法

这是一项前瞻性队列研究，于2024年1月至2024年4月收集了194对IVF夫妇的精液样本。根据卵母细胞成熟度和常规精液分析对患者进行分组。通过流式细胞术检测用于IVF的同一射精精液中的精子NEU1和NEU3，随后进行与受精率的相关性分析。

结果

NEU1和NEU3是独立于常规精液参数的指标。在中期II（MII）率≥80%且精液常规分析正常的情况下，精子NEU1（P = 0.0300）和NEU3（P = 0.0253）水平在受精率为80%时存在显著差异。精子上较低的NEU1和NEU3表达与较低的受精率相关。ROC曲线分析显示NEU1（P = 0.0306，AUC = 0.660）和NEU3（P = 0.0259，AUC = 0.665）的诊断准确性。χ检验分析了NEU1（χ = 4.671，P = 0.0307）或NEU3（χ = 6.377，P = 0.0116）的受精率和不同截断率。