Aromatic and nitrogen-containing acid separation by reversed-phase high performance liquid chromatography and identification by photodiode array detector.

A new method of separation and identification of aromatic and nitrogen-containing acids is presented. Fifty-seven different acid standards were separated by reversed-phase high performance liquid chromatography (HPLC). Detection and identification of the acids by ultraviolet (UV) spectra using a photodiode array detector (DAD) is demonstrated. Peak height ratios are reported and complete UV spectra of these acids are displayed. Possible applications of this technique are demonstrated using patients' urines.