Maier R D, Bogusz M
Institute of Forensic Medicine, Aachen University of Technology, Germany.
J Anal Toxicol. 1995 Mar-Apr;19(2):79-83. doi: 10.1093/jat/19.2.79.
High-performance liquid chromatography with photodiode-array detection (HPLC-DAD) provides two identification parameters: retention and UV spectral data. The identification power of these two parameters, expressed in standardized form (retention index and absorption maximum with the highest wavelength), is calculated using two approaches: discriminating power (DP) and mean list length (MLL). Our own HPLC database, which comprises data for more than 370 substances, is used as the basis of calculations. The identification power of both parameters applied separately is low but increases substantially when the combination of retention and spectral data is applied. Additionally, the DP and MLL values obtained for 56 acidic or neutral and 76 basic drugs examined by means of HPLC-DAD and other analytical methods (thin-layer chromatography, gas chromatography (GC), and ultraviolet (UV) detection) are compared. The on-line combination of HPLC retention index values and UV spectra, registered by means of DAD, creates an identification system in which the identification potential is slightly lower than the off-line combination of capillary GC and UV spectroscopy.
带光电二极管阵列检测的高效液相色谱法（HPLC-DAD）提供两个鉴定参数：保留时间和紫外光谱数据。这两个参数的鉴定能力以标准化形式（保留指数和最大吸收波长）表示，使用两种方法计算：鉴别能力（DP）和平均列表长度（MLL）。我们自己的HPLC数据库包含370多种物质的数据，用作计算基础。单独应用这两个参数时的鉴定能力较低，但当同时应用保留时间和光谱数据时，鉴定能力会大幅提高。此外，还比较了通过HPLC-DAD和其他分析方法（薄层色谱法、气相色谱法（GC）和紫外（UV）检测）检测的56种酸性或中性药物和76种碱性药物的DP和MLL值。通过DAD记录的HPLC保留指数值和紫外光谱的在线组合创建了一个鉴定系统，其鉴定潜力略低于毛细管GC和紫外光谱的离线组合。
