叶酸依赖性转甲酰酶在人骨髓以及大鼠骨髓和肝脏中嘌呤合成中的作用。
Role of folate dependent transformylases in synthesis of purine in bone marrow of man and in bone marrow and liver of rats.
作者信息
Deacon R, Chanarin I, Lumb M, Perry J
出版信息
J Clin Pathol. 1985 Dec;38(12):1349-52. doi: 10.1136/jcp.38.12.1349.
Abstract
The activity of the two folate dependent enzymes supplying carbon 2 and carbon 8 of the purine nucleus was assayed in the bone marrow of rats and man, as well as in rat livers. The activity of both enzymes was several fold greater in marrow than in liver. Inactivation of cobalamin by exposure to nitrous oxide did not affect the enzymes in rat marrow cells, although an appreciable effect on hepatic enzymes was found. The depression of hepatic glycinamide ribotide (GAR) transformylase in rats exposed to nitrous oxide was prevented by supplying a formate precursor, methylthioadenosine. There was a considerable rise in the activity of GAR transformylase in human marrow cells from patients with megaloblastic anaemia due to cobalamin deficiency but no change in activity in marrow from patients deficient in folate.
摘要
在大鼠和人类的骨髓以及大鼠肝脏中，对提供嘌呤核第2位和第8位碳原子的两种叶酸依赖性酶的活性进行了测定。两种酶在骨髓中的活性均比在肝脏中高几倍。暴露于一氧化二氮导致的钴胺素失活对大鼠骨髓细胞中的酶没有影响，尽管发现对肝脏中的酶有明显影响。通过提供甲酸盐前体甲硫腺苷可防止暴露于一氧化二氮的大鼠肝脏甘氨酰胺核苷酸（GAR）转甲酰酶活性降低。钴胺素缺乏所致巨幼细胞贫血患者的人类骨髓细胞中，GAR转甲酰酶活性显著升高，但叶酸缺乏患者的骨髓中该酶活性无变化。
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本文引用的文献
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Purine synthesis de novo in lymphocytes from patients with gout.痛风患者淋巴细胞中嘌呤的从头合成
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