Ma Bo, Miao Yuanxin, Xu Yan, Li Feng, Zhu Deyan, Li Rong, Huang Yechuan
School of Food and Biology, Jingchu University of Technology, Jingmen, PR China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, PR China.
NPJ Sci Food. 2025 Aug 8;9(1):169. doi: 10.1038/s41538-025-00534-4.
This study evaluated the in vitro and in vivo antioxidant activities of puerarin (PUE) and β-lactoglobulin (β-lg). The results of the in vitro antioxidant assay revealed that the DPPH and ABTS radical scavenging rates of the PUE/β-lg complex were generally superior to those of free PUE within the tested experimental concentration range. The in vivo antioxidant activity assay, using Caenorhabditis elegans (C. elegans) as a model organism, showed that the PUE/β-lg complex significantly increased the superoxide dismutase (SOD) activity and reduced glutathione (GSH) content in C. elegans, and also increased the mean lifespan of C. elegans under oxidative and thermal stress conditions. Transcriptomic analysis showed that the PUE/β-lg complex regulated the mRNA expression levels of genes associated with the activation of various signaling pathways, such as the longevity regulation pathway, insulin signaling pathway, and GSH metabolism. Overall, this study demonstrated the potential of the PUE/β-lg complex as an antioxidant, which can lead to its development into food products or pharmaceuticals.
本研究评估了葛根素（PUE）和β-乳球蛋白（β-lg）的体外和体内抗氧化活性。体外抗氧化试验结果表明，在测试的实验浓度范围内，PUE/β-lg复合物的DPPH和ABTS自由基清除率总体上优于游离PUE。以秀丽隐杆线虫（C. elegans）为模式生物的体内抗氧化活性试验表明，PUE/β-lg复合物显著提高了秀丽隐杆线虫中超氧化物歧化酶（SOD）的活性和还原型谷胱甘肽（GSH）的含量，并且在氧化和热应激条件下还延长了秀丽隐杆线虫的平均寿命。转录组分析表明，PUE/β-lg复合物调节了与各种信号通路激活相关基因的mRNA表达水平，如长寿调节通路、胰岛素信号通路和GSH代谢。总体而言，本研究证明了PUE/β-lg复合物作为抗氧化剂的潜力，这可能促使其开发成食品或药品。
