Hosseini J M, Elin R J
J Am Coll Nutr. 1985;4(6):613-7. doi: 10.1080/07315724.1985.10720103.
We determined the magnesium content for two different Burkitt's lymphoma cell lines (EW 36 and CA 46) after transfer to new media for 7 consecutive days. Aliquots of the cell culture were washed and the cell pellet was obtained by centrifugation and lysed with distilled water with and without the addition of 0.25% lanthanum oxide. Magnesium was determined by atomic absorption spectrophotometry. The addition of lanthanum permitted the detection of between 8 and 40% more magnesium. The increased magnesium liberated by the addition of lanthanum was calculated as the "bound" magnesium. The results show that the total magnesium is inversely related and the bound magnesium directly related to the age of the cell culture. Thus, there is a decrease of lymphocyte magnesium content and an increase in the percentage of magnesium bound for these two cell lines with increasing age of the cell culture.
我们连续7天将两种不同的伯基特淋巴瘤细胞系（EW 36和CA 46）转移至新培养基后，测定了其镁含量。取细胞培养物的等分试样进行洗涤，通过离心获得细胞沉淀，然后在添加和不添加0.25%氧化镧的情况下用蒸馏水进行裂解。通过原子吸收分光光度法测定镁含量。添加镧后可检测到的镁含量增加了8%至40%。添加镧后释放出的增加的镁被计算为“结合”镁。结果表明，总镁含量呈负相关，而结合镁与细胞培养的年龄呈正相关。因此，随着这两种细胞系细胞培养年龄的增加，淋巴细胞镁含量降低，镁结合百分比增加。
