Salmonella is a significant zoonotic pathogen affecting both humans and animals. Between April 2023 and May 2024, we obtained 124 Salmonella strains from 1,413 samples (8.78 %) collected from food animals in Weifang, Zibo, Heze, Taian, Linyi, Binzhou, and Jining regions of Shandong Province, China. This study aimed to analyze serovar distribution, multilocus sequence typing (MLST), antimicrobial susceptibility, and plasmid typing of isolated strains. The 124 Salmonella isolates were serotyped into 5 distinct serovars, and S. Typhimurium (58/124, 46.77 %) was the predominant serotype. MLST revealed six distinct sequence types, among which ST19 (40/124, 32.26 %) was the most common. Of the 124 isolates, 83 (66.94 %) displayed multidrug resistance, with ampicillin resistance (88/124, 70.97 %) being the most prevalent phenotype. The bla gene (57/124, 45.97 %) was the most common resistance gene, followed by bla (45/124, 36.29 %), qnrS (39/124, 31.45 %), aph(3')-II (36/124, 29.03 %), oqxA (29/124, 23.39 %), and oqxB (28/124, 22.58 %). These Salmonella strains carried a total of six plasmid incompatibility groups, with IncFII (35/124, 28.23 %) being the most prevalent. Conjugation experiments demonstrated successful transfer of the bla gene from 36 donors (36/57, 63.16 %) to Escherichia coli J53. The spread of resistance genes is largely driven by antibiotic misuse. In conclusion, controlling Salmonella prevalence and limiting antibiotic use in pigs and ducks are crucial for safeguarding public health.