Dai Zhenkai, Liao Liqin, Chen Sheng, Xiao Zhengzhong, Zhang Xinheng, Xie Qingmei
State Key Laboratory of Swine and Poultry Breeding Industry & Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, PR China.
State Key Laboratory of Swine and Poultry Breeding Industry & Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, PR China.
Poult Sci. 2025 Aug 7;104(11):105664. doi: 10.1016/j.psj.2025.105664.
The generation of induced pluripotent stem cells (iPSCs) from somatic cells holds immense promise for regenerative medicine and disease modeling. However, the reprogramming efficiency in avian species, such as chickens, has been notably lower than in mammalian models. This study investigates the potential of microRNAs (miRNAs) to enhance the reprogramming of chicken embryo fibroblasts (CEFs) into iPSCs. We report the identification and utilization of gga-miRNA-302s and the novel gga-miRNA-1811, which were found to be co-expressed during early chicken embryonic development. The combination of these miRNAs with key pluripotency genes significantly improved the reprogramming efficiency of CEFs into iPSCs, resulting in cells with robust pluripotency markers and differentiation potential. Our findings demonstrate that co-expression of gga-miRNA-1811 and gga-miRNA-302s plays a critical role in promoting the mesenchymal-to-epithelial transition, a crucial step in the reprogramming process. These results not only advance the field of avian iPSC generation but also offer a valuable strategy for improving reprogramming efficiency across species, with implications for stem cell research and translational medicine.
从体细胞生成诱导多能干细胞（iPSC）在再生医学和疾病建模方面具有巨大潜力。然而，禽类（如鸡）的重编程效率明显低于哺乳动物模型。本研究调查了微小RNA（miRNA）增强鸡胚成纤维细胞（CEF）重编程为iPSC的潜力。我们报告了gga-miRNA-302s和新型gga-miRNA-1811的鉴定与应用，它们在鸡胚胎早期发育过程中共同表达。这些miRNA与关键多能性基因的组合显著提高了CEF重编程为iPSC的效率，产生了具有强大多能性标记和分化潜能的细胞。我们的研究结果表明，gga-miRNA-1811和gga-miRNA-302s的共同表达在促进间充质向上皮转变中起关键作用，这是重编程过程中的关键步骤。这些结果不仅推动了禽类iPSC生成领域的发展，还为提高跨物种重编程效率提供了有价值的策略，对干细胞研究和转化医学具有重要意义。
