Zhang Wenjun, Yuan Shuoyue, Chen Ruoxin, Li Pengzhen, Huang Qinyu, Zhou Zhenlong, Fan Penghui, Su Jianyu
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; China-Singapore International Joint Research Institute, Guangzhou 510640, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; China-Singapore International Joint Research Institute, Guangzhou 510640, China.
Int J Biol Macromol. 2025 Sep;322(Pt 1):146703. doi: 10.1016/j.ijbiomac.2025.146703. Epub 2025 Aug 8.
Exopolysaccharides derived from endophytic fungi inhabiting medicinal plants are promising bioactive agents. Herein, a neutral exopolysaccharide termed DEPS was extracted and purified from an endophytic fungus isolated from Cinnamomum burmannii leaves, with a yield of 51.12 %. Structural analysis by HPGPC, UV, FTIR, NMR, and SEM revealed that DEPS is primarily composed of glucose, with minor amounts of mannose and galactose, and features →6)-α-d-Glcp-(1→ and →4)-α-d-Glcp-(1→ linkages. Functionally, DEPS exhibited a superior moisture-retention rate of 26.4 %, comparable to sodium alginate and glycerol, and showed moderate antioxidant activity. In vitro, DEPS effectively suppressed IL-6 expression in macrophages by approximately twofold, and inhibited zebrafish neutrophil migration by 50 %. In an acetaminophen (APAP)-induced liver injury model, DEPS treatment significantly alleviated hepatic damage by decreasing necrosis area from 55.2 % to 24.1 %, and reducing IL-1β expression from 273.3 % to 118.6 %. Additionally, DEPS promoted an anti-inflammatory environment through enhanced IL-10 expression and reduced TNF-α levels. These findings highlight DEPS as a structurally unique, multifunctional polysaccharide with hepatoprotective, anti-inflammatory, and antioxidant potential for food, pharmaceutical, and cosmetic applications.
源自药用植物内生真菌的胞外多糖是很有前景的生物活性剂。在此，从阴香叶中分离出的一种内生真菌中提取并纯化了一种名为DEPS的中性胞外多糖，产率为51.12%。通过高效凝胶渗透色谱（HPGPC）、紫外光谱（UV）、傅里叶变换红外光谱（FTIR）、核磁共振（NMR）和扫描电子显微镜（SEM）进行的结构分析表明，DEPS主要由葡萄糖组成，含有少量甘露糖和半乳糖，并具有→6)-α-d-葡萄糖-(1→和→4)-α-d-葡萄糖-(1→连接。在功能方面，DEPS表现出26.4%的优异保湿率，与海藻酸钠和甘油相当，并显示出中等的抗氧化活性。在体外，DEPS能有效抑制巨噬细胞中白细胞介素-6（IL-6）的表达约两倍，并抑制斑马鱼中性粒细胞迁移50%。在对乙酰氨基酚（APAP）诱导的肝损伤模型中，DEPS治疗通过将坏死面积从55.2%降至24.1%，并将IL-1β表达从273.3%降至118.6%，显著减轻了肝损伤。此外，DEPS通过增强IL-10表达和降低肿瘤坏死因子-α（TNF-α）水平促进了抗炎环境。这些发现突出了DEPS作为一种结构独特、多功能的多糖，在食品、制药和化妆品应用中具有肝脏保护、抗炎和抗氧化潜力。
