Leeper H F, Charlton J S
J Neurophysiol. 1985 Nov;54(5):1157-66. doi: 10.1152/jn.1985.54.5.1157.
A new experimental preparation for intracellular recording from mammalian retinal neurons has been established. This is the arterially perfused eyecup preparation of the tree squirrel, Sciurus carolinensis. Two horizontal cell types have been recorded in this preparation. These cell types differ in their response waveforms. Type 1 horizontal cells appear to receive input only from cones, whereas type 2 horizontal cells receive mixed rod-cone input. Sciurus horizontal cells have receptive-field sizes smaller than those of horizontal cells in other mammalian and nonmammalian species. Recordings from a second class of cells in the outer retina of the squirrel reveal receptive-field properties considerably different from those of squirrel horizontal cells and similar to those of photoreceptor cells in other species. On the basis of these receptive-field properties, recording depth, and spectral response characteristics, we conclude that these recordings were from cone-photoreceptor cells. These recordings from squirrel cones are the first demonstration of mammalian photoreceptor response amplitudes and receptive-field interactions comparable with those demonstrated for nonmammalian species.
一种用于从哺乳动物视网膜神经元进行细胞内记录的新实验制备方法已经建立。这是对松鼠（Sciurus carolinensis）进行动脉灌注的眼杯制备方法。在此制备方法中记录到了两种水平细胞类型。这些细胞类型在其反应波形上有所不同。1型水平细胞似乎仅从视锥细胞接收输入，而2型水平细胞接收杆体 - 视锥细胞的混合输入。松鼠的水平细胞的感受野大小比其他哺乳动物和非哺乳动物物种的水平细胞要小。对松鼠外视网膜中另一类细胞的记录显示，其感受野特性与松鼠水平细胞的感受野特性有很大不同，且与其他物种的光感受器细胞的感受野特性相似。基于这些感受野特性、记录深度和光谱反应特征，我们得出结论，这些记录来自视锥光感受器细胞。来自松鼠视锥细胞的这些记录首次证明了哺乳动物光感受器的反应幅度和感受野相互作用与非哺乳动物物种所展示的相当。
