Lorentz K, Flatter B
Enzyme. 1979;24(3):163-8. doi: 10.1159/000458649.
Storage of human serum, saliva and duodenal secretion transformed amylase fractions on cellulose acetate membranes into more anionic forms. Incubation with lectins, proteases, glucosidases, neuraminidase and some effectors did not modify this conversion, which was promoted by rising temperature and pH values. Increasing concentrations of ammonium ions delayed the transformation of amylolytic fractions, thus indicating nonenzymatic deamidation as the reason for isoamylase development. A change of molecular weight could be excluded.
人血清、唾液和十二指肠分泌物中的淀粉酶组分储存在醋酸纤维素膜上时会转变为更多的阴离子形式。与凝集素、蛋白酶、糖苷酶、神经氨酸酶及一些效应物共同孵育并不会改变这种转变，而温度升高和pH值升高会促进这种转变。铵离子浓度增加会延迟淀粉分解组分的转变，因此表明非酶脱酰胺作用是异淀粉酶产生的原因。分子量的变化可以排除。
