James Remya, Vineeth Kumar T V, Koushik Narayan Subramanyam, Payva Febby, Pavithra E Amrisa, Venketesh Sivaramakrishnan, Santhy K S
Department of Zoology, St. Joseph's College for Women, Alappuzha, Kerala, 688001, India.
School of Biosciences, Department of Zoology, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, 614043, India.
Sci Rep. 2025 Aug 11;15(1):29384. doi: 10.1038/s41598-025-07294-x.
D-mannose, a plant-derived monosaccharide used as a dietary supplement, has shown potential in alleviating obesity symptoms and improving bone loss in mice. Obesity, a known risk factor for osteoporosis (OP), suggests shared molecular pathways between these conditions. This study explores the molecular mechanisms of obesity-induced OP and the potential therapeutic role of mannose supplementation. Using in-silico analysis, GEO2R was applied to dataset GSE110796 to identify differentially expressed genes (DEGs) under high-fat diet-induced obesity and mannose supplementation. Enrichment analysis via Enrichr and ClueGO revealed significant molecular pathways altered by high-fat diets and reversed by mannose. Similarly, pathways for OP were identified using the GEO dataset GSE56815 and DisGeNET-associated genes. Forty-four overlapping pathways were identified between obesity and OP, with key immune and inflammatory pathways modulated by mannose. Notably, genes upregulated in osteoclast differentiation during obesity were downregulated with mannose. These findings suggest that mannose modulates shared pathways in obesity and OP, offering a cost-effective therapeutic approach. This study enhances understanding of obesity-induced OP and provides a foundation for innovative medical interventions.
D-甘露糖是一种源自植物的单糖，用作膳食补充剂，已显示出在减轻小鼠肥胖症状和改善骨质流失方面的潜力。肥胖是骨质疏松症（OP）的已知风险因素，表明这些病症之间存在共同的分子途径。本研究探讨了肥胖诱导的OP的分子机制以及甘露糖补充剂的潜在治疗作用。通过计算机分析，将GEO2R应用于数据集GSE110796，以鉴定高脂饮食诱导的肥胖和甘露糖补充下的差异表达基因（DEG）。通过Enrichr和ClueGO进行的富集分析揭示了高脂饮食改变并被甘露糖逆转的重要分子途径。同样，使用GEO数据集GSE56815和DisGeNET相关基因鉴定了OP的途径。在肥胖和OP之间鉴定出44条重叠途径，关键的免疫和炎症途径由甘露糖调节。值得注意的是，肥胖期间破骨细胞分化中上调的基因在使用甘露糖后下调。这些发现表明，甘露糖调节肥胖和OP中的共同途径，提供了一种经济有效的治疗方法。本研究增进了对肥胖诱导的OP的理解，并为创新的医学干预措施提供了基础。
