Creech C Buddy, Leguia Mariana, Goll Johannes B, Howard Leigh M, Vila-Sanjurjo Antón, Yoder Sandra, Juarez Diana, Garcia-Glaessner Alejandra, Gelber Casey E, Jimenez-Truque Natalia, Cherikh Sami, Gil Ana I, Crowe James E, Cotos Rubelio Cornejo, Edwards Kathryn M, Lanata Claudio F
Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
Genomics Laboratory, Pontificia Universidad Católica del Perú (PUCP), Lima, Peru.
NPJ Vaccines. 2025 Aug 11;10(1):189. doi: 10.1038/s41541-025-01170-5.
Despite robust antibody responses, immunity induced by acellular pertussis vaccine (DTaP) wanes over time and risk of pertussis seems to be lower in children who receive whole-cell vaccine (DTP) as their first dose. To interrogate the early immunologic response to pertussis vaccine, we enrolled 56 healthy infants who received either DTP or DTaP at 2-, 4-, 6-, and 18-months of age. RNA-sequencing and ribosome profiling of PBMC were performed prior to vaccination (Day 1) and on either Day 2 or Day 8. Pathway enrichment analysis on Days 2 and 8 showed enrichment of TLR-signaling and FcϒR-mediated phagocytosis among DTP recipients. DTP also led to increases in IRAK-4 and IL-1ß. After booster vaccination, a higher frequency of PT-specific B-cells was observed in DTP- vs. DTaP recipients. These data provide insights into the early immunologic responses to pertussis vaccine and may guide next-generation pertussis vaccine development.
尽管有强大的抗体反应，但无细胞百日咳疫苗（DTaP）诱导的免疫力会随着时间推移而减弱，而首剂接种全细胞疫苗（DTP）的儿童患百日咳的风险似乎更低。为了探究对百日咳疫苗的早期免疫反应，我们招募了56名健康婴儿，他们在2个月、4个月、6个月和18个月时分别接种了DTP或DTaP。在接种疫苗前（第1天）以及第2天或第8天对PBMC进行了RNA测序和核糖体分析。第2天和第8天的通路富集分析显示，DTP接种者中TLR信号传导和FcϒR介导的吞噬作用有所富集。DTP还导致IRAK-4和IL-1ß增加。加强免疫后，DTP接种者中PT特异性B细胞的频率高于DTaP接种者。这些数据为百日咳疫苗的早期免疫反应提供了见解，并可能指导下一代百日咳疫苗的开发。
