婴儿对全细胞百日咳疫苗和无细胞百日咳疫苗免疫反应的免疫谱分析。
Immunologic profiling of the infant immune response to whole-cell and acellular pertussis vaccines.
作者信息
Creech C Buddy, Leguia Mariana, Goll Johannes B, Howard Leigh M, Vila-Sanjurjo Antón, Yoder Sandra, Juarez Diana, Garcia-Glaessner Alejandra, Gelber Casey E, Jimenez-Truque Natalia, Cherikh Sami, Gil Ana I, Crowe James E, Cotos Rubelio Cornejo, Edwards Kathryn M, Lanata Claudio F
机构信息
Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
Genomics Laboratory, Pontificia Universidad Católica del Perú (PUCP), Lima, Peru.
出版信息
NPJ Vaccines. 2025 Aug 11;10(1):189. doi: 10.1038/s41541-025-01170-5.
Despite robust antibody responses, immunity induced by acellular pertussis vaccine (DTaP) wanes over time and risk of pertussis seems to be lower in children who receive whole-cell vaccine (DTP) as their first dose. To interrogate the early immunologic response to pertussis vaccine, we enrolled 56 healthy infants who received either DTP or DTaP at 2-, 4-, 6-, and 18-months of age. RNA-sequencing and ribosome profiling of PBMC were performed prior to vaccination (Day 1) and on either Day 2 or Day 8. Pathway enrichment analysis on Days 2 and 8 showed enrichment of TLR-signaling and FcϒR-mediated phagocytosis among DTP recipients. DTP also led to increases in IRAK-4 and IL-1ß. After booster vaccination, a higher frequency of PT-specific B-cells was observed in DTP- vs. DTaP recipients. These data provide insights into the early immunologic responses to pertussis vaccine and may guide next-generation pertussis vaccine development.
尽管有强大的抗体反应，但无细胞百日咳疫苗（DTaP）诱导的免疫力会随着时间推移而减弱，而首剂接种全细胞疫苗（DTP）的儿童患百日咳的风险似乎更低。为了探究对百日咳疫苗的早期免疫反应，我们招募了56名健康婴儿，他们在2个月、4个月、6个月和18个月时分别接种了DTP或DTaP。在接种疫苗前（第1天）以及第2天或第8天对PBMC进行了RNA测序和核糖体分析。第2天和第8天的通路富集分析显示，DTP接种者中TLR信号传导和FcϒR介导的吞噬作用有所富集。DTP还导致IRAK-4和IL-1ß增加。加强免疫后，DTP接种者中PT特异性B细胞的频率高于DTaP接种者。这些数据为百日咳疫苗的早期免疫反应提供了见解，并可能指导下一代百日咳疫苗的开发。
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