Davias Aline, Lyon-Caen Sarah, Iszatt Nina, Monot Celine, Rayah Yamina, Ilhan Zehra Esra, Guichardet Karine, Bayat Sam, Valmary-Degano Séverine, Muckle Gina, Eggesbø Merete, Lepage Patricia, Philippat Claire, Slama Rémy
Environmental Epidemiology applied to Development and Respiratory Health team, Institute for Advanced Biosciences, University Grenoble Alpes, Inserm, CNRS, Grenoble, France.
Division of Climate and Environmental Health, Norwegian Institute of Public Health (NIPH), 0213, Oslo, Norway.
Brain Behav Immun Health. 2025 Jul 18;48:101063. doi: 10.1016/j.bbih.2025.101063. eCollection 2025 Oct.
Fundamental research indicates a communication between the gut microbiota and the central nervous system, referred to as the microbiota-gut-brain axis. This link is little characterized in humans in the general population. We prospectively investigated the relationships between the gut microbiota composition of one-year-old children and neurodevelopment parameters at 2 and 3 years of age. Within the SEPAGES French couple-child cohort, we profiled gut microbiota by sequencing the V3-V4 region of the 16S rRNA gene from stool samples in 356 children at 12 months of age. We later assessed children's neurodevelopment through validated tests (CBCL at 2 years, and SRS-2, BRIEF-P and WPPSI-IV at 3 years). Microbial α-diversity indices, the 4 most abundant phyla, and the 46 most abundant genera were analyzed for their relations with neurodevelopmental parameters using multiple linear regression, while associations of β-diversity with neurodevelopment were examined through PERMANOVA tests. α- and β-diversity indices were not associated with neurodevelopmental parameters in children. Suggestive associations were observed with taxonomy, but not maintained after correction for multiple comparisons: phyla Proteobacteria and Bacteroidetes tended to be associated with higher socio-emotional neurodevelopment assessed with different sub-scores; phylum Firmicutes with increased plan and organization problems; genera , , and with lower socio-emotional neurodevelopment, while genera and tended to be associated with higher socio-emotional neurodevelopment, assessed with different sub-scores. Within this generally healthy population, only suggestive associations were observed between gut microbiota composition and neurodevelopmental scores at 2 and 3 years. Larger studies are needed to examine a possibly weak link between the gut microbiota of one-year-old children and their neurodevelopment.
基础研究表明肠道微生物群与中枢神经系统之间存在一种联系，即微生物群-肠道-脑轴。在普通人群中，这种联系在人类中的特征鲜为人知。我们前瞻性地研究了一岁儿童的肠道微生物群组成与2岁和3岁时神经发育参数之间的关系。在SEPAGES法国夫妇-子女队列中，我们通过对356名12个月大儿童粪便样本中的16S rRNA基因V3-V4区域进行测序，分析了肠道微生物群。随后，我们通过经过验证的测试（2岁时的CBCL，3岁时的SRS-2、BRIEF-P和WPPSI-IV）评估了儿童的神经发育。使用多元线性回归分析了微生物α多样性指数、4个最丰富的门和46个最丰富的属与神经发育参数之间的关系，同时通过PERMANOVA测试检验了β多样性与神经发育的关联。α和β多样性指数与儿童的神经发育参数无关。在分类学上观察到了一些提示性关联，但在进行多重比较校正后并未保持：变形菌门和拟杆菌门往往与用不同子分数评估的较高社会情感神经发育相关；厚壁菌门与计划和组织问题增加相关；属、、和与较低的社会情感神经发育相关，而属和往往与用不同子分数评估的较高社会情感神经发育相关。在这个总体健康的人群中，仅在2岁和3岁时观察到肠道微生物群组成与神经发育分数之间存在提示性关联。需要进行更大规模的研究来检验一岁儿童的肠道微生物群与其神经发育之间可能存在的微弱联系。
