铜绿假单胞菌和睾丸酮假单胞菌中的嘌呤降解

Purine degradation in Pseudomonas aeruginosa and Pseudomonas testosteroni.

Bongaerts G P, Sin I L, Peters A L, Vogels G D

Biochim Biophys Acta. 1977 Aug 25;499(1):111-8. doi: 10.1016/0304-4165(77)90233-1.

Abstract

Adenine, hypoxanthine, xanthine and guanine are broken down in Pseudomonas aeruginosa and Pseudomonas testosteroni to allantoin by the concerted action of the enzymes adenine deaminase, guanine deaminase, NAD+-dependent xanthine dehydrogenase and uricase. 2. Uric acid is broken down by an unstable, membrane-bound uricase with an unusually low pH optimum. 3. In both strains adenine inhibits growth and xanthine dehydrogenase. A second type of inhibition is manifest only in Ps. testosteroni and concerns the regulation of the biosynthesis of amino acids of the aspartate family. Enzymic studies showed that in this strain aspartate kinase is inhibited by AMP.

摘要

在铜绿假单胞菌和睾丸酮假单胞菌中，腺嘌呤、次黄嘌呤、黄嘌呤和鸟嘌呤通过腺嘌呤脱氨酶、鸟嘌呤脱氨酶、NAD⁺依赖性黄嘌呤脱氢酶和尿酸酶的协同作用被分解为尿囊素。2. 尿酸由一种不稳定的、膜结合的尿酸酶分解，该酶的最适pH值异常低。3. 在这两种菌株中，腺嘌呤均抑制生长和黄嘌呤脱氢酶。第二种抑制类型仅在睾丸酮假单胞菌中表现出来，涉及天冬氨酸家族氨基酸生物合成的调节。酶学研究表明，在该菌株中，天冬氨酸激酶被AMP抑制。