Csicsaky M, Papadopoulos R, Wiegand H
J Neurosci Methods. 1985 Oct-Nov;15(2):113-29. doi: 10.1016/0165-0270(85)90049-4.
In phrenic nerve-diaphragm preparations from young rats and mice, long-term recordings of miniature endplate potentials (MEPPs) from single synapses were performed. Amplitude histograms obtained from these experiments were analysed with respect to the presence of multiple peaks which are believed to reflect quantal transmitter release. This was accomplished by subjecting MEPP amplitude histograms as well as matched random number histograms to Fourier analysis; harmonics in the MEPP amplitude distribution were considered valid only if they exceeded those of the random number distribution. In 9 out of 11 experiments, substantial evidence for the existence of preferred amplitudes was obtained; in two cases, however, it was completely missing. Applying our method to a series of promising MEPP amplitude histograms selected from the literature, we found a similar relationship between positive and negative results. We also noted a fairly constant relationship between the size of the elementary sub-MEPPs and the mean amplitude of the bell-MEPPs, named so according to the Gaussian shape of their amplitude distribution.
在来自幼鼠和小鼠的膈神经-膈肌标本中，对单个突触的微小终板电位（MEPPs）进行了长期记录。针对这些实验获得的幅度直方图，分析了多个峰值的存在情况，这些峰值被认为反映了量子化递质释放。这是通过对MEPP幅度直方图以及匹配的随机数直方图进行傅里叶分析来实现的；仅当MEPP幅度分布中的谐波超过随机数分布中的谐波时，才认为其有效。在11个实验中的9个实验中，获得了存在优先幅度的充分证据；然而，在两个案例中，完全没有发现这种证据。将我们的方法应用于从文献中选出的一系列有前景的MEPP幅度直方图时，我们在阳性和阴性结果之间发现了类似的关系。我们还注意到基本亚MEPPs的大小与钟形MEPPs的平均幅度之间存在相当恒定的关系，钟形MEPPs因其幅度分布的高斯形状而得名。
