温度和紫外线照射对噬菌体phi29增殖的影响。
The effects of temperature and ultraviolet irradiation on multiplication of bacteriophage phi29.
作者信息
Larcom L L, Thaker N H
出版信息
Biophys J. 1977 Sep;19(3):299-306. doi: 10.1016/S0006-3495(77)85589-6.
The effects of temperature and of ultraviolet radiation on the multiplication of bacteriophage phi29 were studied. Samples of phi29 that had been irradiated to surviving fractions of 0.44 or 0.10 were propagated at 37 degrees C, 42 degrees C and 43.5 degrees C. Latent periods and burst sizes were obtained from one-step growth curves. At a particular temperature, as the dose delivered to the virus was increased, the latent period was extended and the burst size was decreased. For unirradiated virus, the burst size was the same at 42 degrees C as at 37 degrees C, but decreased dramatically at 43.5 degrees C. For virus subjected to a particular dose, the burst size decreased as the temperature was raised. A statistical technique for improving the reliability of parameters obtained from one-step growth curves is presented.
研究了温度和紫外线辐射对噬菌体φ29增殖的影响。将已辐照至存活分数为0.44或0.10的φ29样品在37℃、42℃和43.5℃下进行增殖。从一步生长曲线获得潜伏期和裂解量。在特定温度下，随着给予病毒的剂量增加，潜伏期延长，裂解量减少。对于未辐照的病毒，42℃时的裂解量与37℃时相同，但在43.5℃时显著降低。对于接受特定剂量的病毒，裂解量随着温度升高而降低。提出了一种提高从一步生长曲线获得的参数可靠性的统计技术。
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