Zhang Qingqing, Zhai Tingting, Chen Jie, Chen Zhongxiu, Tan Hao, Long Lifen, Hao Tingting, Guo Zhiyong
State Key Laboratory for Quality and Safety of Agro-Products, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou 310035, PR China.
Bioelectrochemistry. 2026 Feb;167:109075. doi: 10.1016/j.bioelechem.2025.109075. Epub 2025 Aug 6.
Here, a photoelectrochemical (PEC)/photothermal (PT) dual-mode biosensing platform with simultaneous output of response signals was developed for sensitive and accurate detection of bladder tumor cells HT-1376. The system utilized an infrared laser as a shared light source, with photocurrent and temperature measurements acquired through parallel PEC and PT detection channels. The HT-1376-specific aptamer (Apt) was covalently immobilized on aminated indium‑tin oxide (ITO) electrode via glutaraldehyde crosslinking between surface amino groups and amine-modified aptamer terminals, enabling selective target capture. Following aptamer-HT-1376 recognition, the dual-mode tags CuO@NPC functionalized with aptamer (CuO@NPC-Apt) were assembled on the electrode surface, triggering simultaneous PEC and PT signal responses. Under optimized conditions, linear detection ranges of 5-10 cells/mL (PEC mode) and 10-10 cells/mL (PT mode) were achieved, with limits of detection (LOD) of 3 cells/mL (PEC) and 5 cells/mL (PT). The platform exhibited excellent signal stability, repeatability and selectivity. This dual-mode strategy provides a reliable analytical method for HT-1376 quantification, demonstrating potential for clinical cancer diagnostics.
在此，开发了一种具有响应信号同步输出的光电化学（PEC）/光热（PT）双模式生物传感平台，用于灵敏且准确地检测膀胱肿瘤细胞HT - 1376。该系统利用红外激光作为共享光源，通过并行的PEC和PT检测通道获取光电流和温度测量值。HT - 1376特异性适配体（Apt）通过表面氨基与胺修饰的适配体末端之间的戊二醛交联，共价固定在胺化氧化铟锡（ITO）电极上，实现对目标的选择性捕获。在适配体与HT - 1376识别后，用适配体功能化的双模式标签CuO@NPC（CuO@NPC - Apt）组装在电极表面，引发PEC和PT信号的同步响应。在优化条件下，实现了5 - 10⁴细胞/mL（PEC模式）和10 - 10⁵细胞/mL（PT模式）的线性检测范围，检测限（LOD）分别为3细胞/mL（PEC）和5细胞/mL（PT）。该平台表现出优异的信号稳定性、重复性和选择性。这种双模式策略为HT - 1376定量提供了一种可靠的分析方法，展示了其在临床癌症诊断中的潜力。
