Webber R J, Malemud C J, Sokoloff L
Calcif Tissue Res. 1977 May 31;23(1):61-6. doi: 10.1007/BF02012767.
Articular chondrocytes from eight mammalian species (rabbit, opossum, woodchuck, cat, dog, sheep, rhesus and cebus monkeys) were grown in monolayer culture using a single regimen. The animals were immature or young adults. Ham's F12 medium supplemented with 10% fetal bovine serum was employed for the primary cultures and Dulbecco-Vogt medium, for the secondary. Marked species differences were found with respect to cell morphology, growth in primary and secondary cultures, incorporation of radiosulfate into macromolecules, adhesion to the flask surface, response to vitamin C, and chondroid expression in spinner bottles. Under these particular conditions, rabbit chondrocytes grew most rapidly and incorporated several times more sulfate than did the others. Additional experiments carried out with other media on four of the species indicate that optimal conditions for culturing mammalian chondrocytes must be determined for each species individually.
采用单一培养方案，对8种哺乳动物（兔子、负鼠、土拨鼠、猫、狗、绵羊、恒河猴和松鼠猴）的关节软骨细胞进行单层培养。这些动物均为未成年或年轻成年个体。原代培养使用添加10%胎牛血清的Ham's F12培养基，传代培养则使用杜尔贝科-沃格特培养基。在细胞形态、原代和传代培养中的生长情况、放射性硫酸盐掺入大分子的情况、对培养瓶表面的黏附性、对维生素C的反应以及在旋转瓶中的类软骨表达方面，均发现了显著的物种差异。在这些特定条件下，兔软骨细胞生长最为迅速，硫酸盐掺入量比其他物种高出数倍。对其中4个物种使用其他培养基进行的额外实验表明，必须针对每个物种分别确定培养哺乳动物软骨细胞的最佳条件。
