Bogdanova E S
Mol Biol (Mosk). 1985 Sep-Oct;19(5):1251-8.
We studied nucleosome assemble in vitro in a system containing the relaxed DNA CoIE1, core histones and a crude extract Drosophila embryos. Supercoiling is a criterium for making conclusions about forming nucleosomes. Supercoiling raises more if nucleosome assemble takes place in the presence of histone H1, polylysine of the 20 000 molecular weight or spermine. These agents do not stimulate the relaxation, and they are more effective when they are added earlier. Thus histone H1, spermine and polylysine can facilitate nucleosome assemble in vitro and with two former agents it may be possibly that the same process takes place in vivo.
我们在一个包含松弛型DNA CoIE1、核心组蛋白和果蝇胚胎粗提物的体外系统中研究了核小体组装。超螺旋是判断核小体形成的一个标准。如果在组蛋白H1、20000分子量的聚赖氨酸或精胺存在的情况下发生核小体组装，超螺旋增加得更多。这些物质不会刺激松弛，并且如果更早添加会更有效。因此，组蛋白H1、精胺和聚赖氨酸可以在体外促进核小体组装，并且对于前两种物质，体内可能发生相同的过程。
